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Some Effects Of Ph, Temperature And Light On The Production Of Zoosporagia In Saprolegnia Parasitica Coker, Philip C. Lee

Master's Theses

Pure cultures of Saprolegnia parasitica Coker were grown under varied conditions of temperature, pH, and light conditions. In liquid medium colonies were spherical with a zonal effect in the distribution of zoosporangia.

Near the limiting extremes of pH and temperature, beaded hyphal tips were found in place of normal sporangia. Teorell Universal buffer in the sporulation water inhibited production of sporangia. The pH minimum was 4.0 - 4.1, optimum 6.3 - 7.4, and maximum 8.o - 8.3. The maximum number of sporangia was produced at 15 C. Between 3.4 and 5 C , a delay in sporulation was noted and …