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No Difference Found In Ribosomal Dna Sequences From Physiologically Diverse Clones Of Karenia Brevis (Dinophyceae) From The Gulf Of Mexico, Torstein Tengs
No Difference Found In Ribosomal Dna Sequences From Physiologically Diverse Clones Of Karenia Brevis (Dinophyceae) From The Gulf Of Mexico, Torstein Tengs
Dr. Torstein Tengs
Maximum growth rate and toxin content were significantly different among five strains of Karenia brevis isolated from Texas and Florida when grown under identical culture conditions. Sequence analysis of the 18S rRNA gene and internal transcribed spacer (ITS) regions revealed, however, that all five strains were identical. Consequently, a clear genetic basis for physiological variability among various geographical isolates of K. brevis from the Gulf of Mexico could not be assessed using these genetic markers. Both the ITS and 18S rRNA regions may be useful in species-specific probe selection. At the intra-specific level, however, an alternative marker will be needed …
Discovery Of The Toxic Dinoflagellate Pfiesteria In Northern European Waters, Torstein Tengs
Discovery Of The Toxic Dinoflagellate Pfiesteria In Northern European Waters, Torstein Tengs
Dr. Torstein Tengs
Several dinoflagellate strains of the genus Pfiesteria were isolated by culturing techniques from sediment samples taken in the Oslofjord region of Norway. Pfiesteria piscicida, well known as a fish killer from the Atlantic coast of America, was identified by genetic methods and light microscopy. The related species Pfiesteria shumwayae was attracted from the sediment by the presence of fish, and has proved toxic. This present survey demonstrates the wide distribution of these potentially harmful species, but so far they have not been connected with fish kills in Europe.
Heteroduplex Mobility Assay-Guided Sequence Discovery: Elucidation Of The Small Subunit (18s) Rdna Sequences Of Pfiesteria Piscicida And Related Dinoflagellates From Complex Algal Culture And Environmental Sample Dna Pools, Torstein Tengs
Dr. Torstein Tengs
The newly described heterotrophic estuarine dinoflagellate Pfiesteria piscicida has been linked with fish kills in field and laboratory settings, and with a novel clinical syndrome of impaired cognition and memory disturbance among humans after presumptive toxin exposure. As a result, there is a pressing need to better characterize the organism and these associations. Advances in Pfiesteria research have been hampered, however, by the absence of genomic sequence data. We employed a sequencing strategy directed by heteroduplex mobility assay to detect Pfiesteria piscicida 18S rDNA ‘‘signature’’ sequences in complex pools of DNA and used those data as the basis for determination …