Open Access. Powered by Scholars. Published by Universities.®

Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 3 of 3

Full-Text Articles in Biology

Pubspectra - Open Data Access Fluorescence Spectra, George Mcnamara Feb 2012

Pubspectra - Open Data Access Fluorescence Spectra, George Mcnamara

George McNamara

The Internet is enabling greater access to spectral imaging publications, spectral graphs, and data than that was available a generation ago. The spectral imaging systems discussed in this issue of Cytometry work because reagent and hardware spectra are reproducible, reusable, and provide input to spectral unmixing and spectral components recognition algorithms. These spectra need to be readily available in order to determine what to purchase, how to use it, and what the output means. We refer to several commercially sponsored and academic spectral web sites and discuss our spectral graphing and data sites. Sites include fluorescent dye graph servers from …


Photoperiod Effects On 2n Pollen Production, Response To Anther Culture, And Net Photosynthesis Of A Diplandrous Clone Of Solanum Phureja, Henry Owen, R. Veilleux, F. Haynes, K. Haynes Jan 1988

Photoperiod Effects On 2n Pollen Production, Response To Anther Culture, And Net Photosynthesis Of A Diplandrous Clone Of Solanum Phureja, Henry Owen, R. Veilleux, F. Haynes, K. Haynes

Henry R. Owen

Flow-cytometric analysis of pollen samples obtained from Solanum phureja, clone PP5, grown under 10, 14, and 18 hour photoperiods at the Southeastern Plant Environment Laboratories of North Carolina State University yielded two populations of pollen based on size parameters. These populations corresponded to pollen separation based on propidium iodide staining of DNA. Anther culture response from plants grown under 14 and 18 hour photo periods for 8, I 0, 12, and 14 weeks was greatest from 8 week old plants grown under a 14 hour photoperiod. Net photosynthesis was significantly higher for plants grown under a 10 hour photoperiod than …


Environmental, Genotypic, And Ploidy Effects On Endopolyploidization Within A Genotype Of Solanum Phureja And Its Derivatives, Henry R. Owen, R. E. Veilleux, D. Levy, D. L. Ochs Jan 1988

Environmental, Genotypic, And Ploidy Effects On Endopolyploidization Within A Genotype Of Solanum Phureja And Its Derivatives, Henry R. Owen, R. E. Veilleux, D. Levy, D. L. Ochs

Henry R. Owen

Flow-cytometric analyses of DNA content were performed on chopped in vivo, in vitro, and protoplast-derived in vitro nuclei of Solanum phureja. An anther-derived monoploid genotype and a diploid and tetraploid clone, derived from callus culture of the monoploid genotype, were used to characterize the influence of in vivo and in vitro environment and explant ploidy level on the extent of endopolyploidization. In addition, protoplast-derived nuclei, from nine anther-derived monoploid genotypes, were examined for genotypic influences on endopolyploidization. DNA distributions of the anther-derived monoploid and callus-derived clones in vivo contained peaks corresponding to 1C, 2C, and 4C DNA levels. By comparison, …