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Time Series Data For 3b Image Processing, George Mcnamara
Time Series Data For 3b Image Processing, George Mcnamara
George McNamara
Time series data for 3B image processing
Four time series data sets of Stellaris single molecules RNA FISH (fluorescence in situ hybridization). All four datasets are 301 imaqe planes. FISH probes are to TOP1 mRNA (Topoisomerase I)in Saos osterosarcoma cells.
One dataset is 10 millisecond exposures (very dim), acquired in Streaming acquisition mode (no hardware overhead).
The other three datasets are a three consecutive planes of the same XY field of view.
I have included image processing results for:
PiMP - a fast method developed by Sebastian Munck et al 2012, http://www.ncbi.nlm.nih.gov/pubmed/?term=22357945
and greatly improved performance by Glen Macdonald (Seattle). …
Stellaris Fishing 20131125mon Part 1 Of 2, George Mcnamara
Stellaris Fishing 20131125mon Part 1 Of 2, George Mcnamara
George McNamara
Stellaris FISH dataset using three FISH probe sets. Slides courtesy of Biosearch Technologies,
https://www.biosearchtech.com/store/product.aspx?catid=224,318,324
see http://stellarisfish.smugmug.com/ for online gallery by Biosearch.
This experiment was to evaluate the crosstalk between the Biosearch fluorophores:
Quasar 570
CAL Fluor Red 610 (CFR 610)
Quasar 670
DAPI (DNA counterstain)
Autofluorescence (green, but sometimes showing up in other channels).
and our lab's Leica DMI6000 fluorescence microscope with Leica filter sets:
DAPI
GFP (L5)
Cy3 (N3)
Texas Red (TxRed2)
Cy5 (Y5)
I also acquired green channel and red channel with exciter filters in our ASI excitation wheel:
GFP + 492 exciter
Texas Red (TxRed2) + 572 …
Stellaris Fishing 20131125mon Part 2 Of 2, George Mcnamara
Stellaris Fishing 20131125mon Part 2 Of 2, George Mcnamara
George McNamara
Stellaris FISH dataset using three FISH probe sets.
Slides courtesy of Biosearch Technologies,
https://www.biosearchtech.com/store/product.aspx?catid=224,318,324
see http://stellarisfish.smugmug.com/ for online gallery by Biosearch.
This experiment was to evaluate the crosstalk between the Biosearch fluorophores:
Quasar 570
CAL Fluor Red 610 (CFR 610)
Quasar 670
DAPI (DNA counterstain)
Autofluorescence (green, but sometimes showing up in other channels).
and our lab's Leica DMI6000 fluorescence microscope with Leica filter sets:
DAPI
GFP (L5)
Cy3 (N3)
Texas Red (TxRed2)
Cy5 (Y5)
I also acquired green channel and red channel with exciter filters in our ASI excitation wheel:
GFP + 492 exciter
Texas Red (TxRed2) + 572 …