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Full-Text Articles in Biology

Real-Time Pcr And Real-Time Rt-Pcr Applications In Food Labelling And Gene Expression Studies, Arash Kashani, Tasmanian Institute Of Agriculture Apr 2014

Real-Time Pcr And Real-Time Rt-Pcr Applications In Food Labelling And Gene Expression Studies, Arash Kashani, Tasmanian Institute Of Agriculture

Emily Scott

Polymerase chain reaction (PCR) as a scientific invention, has revolutionized molecular biology and led to real-time PCR and later, real-time reverse transcription PCR (Real-Time RT-PCR). These two techniques enable scientists to conduct PCR detection of amplified gene products and expression analysis of targeted genes. Quantitative polymerase chain reaction (qPCR), also called real-time polymerase chain reaction, is a recent modification to PCR that utilizes fluorescent reporter molecular techniques to monitor the production of amplified products during each cycle of the PCR reaction, and enables both detection and quantification of specific sequences in complex mixtures. Over the past decade, real-time PCR applications …


Mechanisms Of Photoperiod In Regulation Of Rice Flowering, Abdulrahman Mahmoud Dogara, Salisu Muhammad Tahir, Idris Shehu, Mustapha Abbah Mar 2014

Mechanisms Of Photoperiod In Regulation Of Rice Flowering, Abdulrahman Mahmoud Dogara, Salisu Muhammad Tahir, Idris Shehu, Mustapha Abbah

Emily Scott

A study on the photoperiodic control of flower in rice is advancing and rice has become an example of short day plant. Many genes used in flowering time determination in rice have been identified by many methods. The conclusion from these molecular studies is a remarkable conservation of genes which play an important role in the control of flowering time in rice. The rice photoperiod sensitivity gene Hd3a was originally detected as a heading date related quantitative trait locus found on chromosome 6 of rice. High resolution linkage mapping of Hd3a was carried using a huge segregating population derived from …