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Full-Text Articles in Biology
The Role Of Med12 In Adipogenesis, Caroline Helen Rinderle
The Role Of Med12 In Adipogenesis, Caroline Helen Rinderle
Master's Theses
In order for any function to occur within a cell, transcription factors must be able to interact with genes. When this occurs, genes are expressed, and ultimately, proteins are translated and perform the specific function that needs to be done within the cell. In order for this to occur, genes must interact with transcription machinery. The Mediator complex recruits transcription factors to genes in order to promote cell-type specific gene expression. The Mediator complex is a multi-protein complex consisting of four modules: head, middle, tail, and kinase. The kinase module is known to dissociate from the rest of the complex …
The Role Of Med31 And Med12 In Directing Adipogenesis Of Human Adult-Derived Mesenchymal Stem Cells, Joseph Straub
The Role Of Med31 And Med12 In Directing Adipogenesis Of Human Adult-Derived Mesenchymal Stem Cells, Joseph Straub
Master's Theses
Selective gene expression is crucial in maintaining the self-renewing and multipotent properties of stem cells. Mediator is a large, evolutionarily conserved, multisubunit protein complex that modulates gene expression by relaying signals from cell type-specific transcription factors to RNA polymerase II. In humans, this complex consists of 30 subunits arranged in four modules: head, middle, tail, and kinase. In our introduction, we show the state of the field of Mediator study with a focus on the critical kinase module. In the following chapters, we used siRNA knockdowns to investigate the roles of the highly-conserved core subunit MED31 and the kinase module …
The Role Of Med31 And Med12 In Directing Adipogenesis Of Human Adult-Derived Mesenchymal Stem Cells, Joseph Straub
The Role Of Med31 And Med12 In Directing Adipogenesis Of Human Adult-Derived Mesenchymal Stem Cells, Joseph Straub
Doctoral Dissertations
Selective gene expression is crucial in maintaining the self-renewing and multipotent properties of stem cells. Mediator is a large, evolutionarily conserved, multisubunit protein complex that modulates gene expression by relaying signals from cell type-specific transcription factors to RNA polymerase II. In humans, this complex consists of 30 subunits arranged in four modules: head, middle, tail, and kinase. In our introduction, we show the state of the field of Mediator study with a focus on the critical kinase module. In the following chapters, we used siRNA knockdowns to investigate the roles of the highly-conserved core subunit MED31 and the kinase module …
The Role Of Med12 In Adipogenesis Of Human Adipose Stem Cells, Onyekachi Chinelo Idigo
The Role Of Med12 In Adipogenesis Of Human Adipose Stem Cells, Onyekachi Chinelo Idigo
Master's Theses
The interest in adipogenesis stems from the high rate of obesity in the world and the motivation to better understand the process of cell fate in relation to human diseases. Mutations in MED12 lead to developmental disorders and certain malignancies. To date, specific roles of Mediator and MED12 in cell state regulation are unclear, nevertheless, they are critical for understanding stem cell regulation and enhancement of their clinical applications. This study investigates the role of MED12 in relation to the Mediator complex kinase domain to better understand transcriptional control of adipogenesis in hASCs. In this study, MED12 expression was diminished …