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Full-Text Articles in Biology

Preliminary Pcr-Based Screening Indicates A Higher Incidence Of Porcine Endogenous Retrovirus Subtype C (Perv-C) In Feral Versus Domestic Swine, Rashmi Acharya, Zoey K. Wallis, Robert Keener, Eric T. Gillock Ph.D. Jan 2019

Preliminary Pcr-Based Screening Indicates A Higher Incidence Of Porcine Endogenous Retrovirus Subtype C (Perv-C) In Feral Versus Domestic Swine, Rashmi Acharya, Zoey K. Wallis, Robert Keener, Eric T. Gillock Ph.D.

Biological Sciences Faculty Publications

Xenotransplantation is considered a potential alternative to allotransplantation to relieve the current shortage of human organs. Due to their similar size and physiology, the organs of pigs are of particular interest for this purpose. Endogenous retroviruses are a result of integration of retroviral genomes into the genome of infected germ cells as DNA proviruses, which are then carried in all cells of the offspring of the organism. Porcine endogenous retroviruses (PERVs) are of special concern because they are found in pig organs and tissues that might otherwise be used for xenotransplantation. PERV proviruses can be induced to replicate and recombine …


Local Adaptation, Genetic Divergence, And Experimental Selection In A Foundation Grass Across The Us Great Plains’ Climate Gradient, Matthew Galliart, Nora Bello, Mary Knapp, Jesse Poland, Paul St Amand, Sara Baer, Brian Maricle Ph.D., Adam B. Smith, Loretta Johnson Jan 2019

Local Adaptation, Genetic Divergence, And Experimental Selection In A Foundation Grass Across The Us Great Plains’ Climate Gradient, Matthew Galliart, Nora Bello, Mary Knapp, Jesse Poland, Paul St Amand, Sara Baer, Brian Maricle Ph.D., Adam B. Smith, Loretta Johnson

Biological Sciences Faculty Publications

Many prior studies have uncovered evidence for local adaptation using reciprocal transplant experiments. However, these studies are rarely conducted for a long enough time to observe succession and competitive dynamics in a community context, limiting inferences for long-lived species. Furthermore, the genetic basis of local adaptation and genetic associations with climate has rarely been identified. Here, we report on a long-term (6-year) experiment conducted under natural conditions focused on Andropogon gerardii, the dominant grass of the North American Great Plains tallgrass ecosystem. We focus on this foundation grass that comprises 80% of tallgrass prairie biomass and is widely used in …


The Cure For Cultivating Fastidious Microbes, Arundhati Bakshi, Austen T. Webber, Lorelei E. Patrick, William Wischusen, Cameron Thrash Jan 2019

The Cure For Cultivating Fastidious Microbes, Arundhati Bakshi, Austen T. Webber, Lorelei E. Patrick, William Wischusen, Cameron Thrash

Biological Sciences Faculty Publications

Course-Based Undergraduate Research Experiences (CUREs) expand the scientific educational benefits of research to large groups of students in a course setting. As part of an ongoing effort to integrate CUREs into first-year biology labs, we developed a microbiology CURE (mCURE) that uses a modified dilution-toextinction high throughput culturing protocol for isolating abundant yet fastidious aquatic bacterioplankton during one semester. Students learn common molecular biology techniques like nucleic acid extraction, PCR, and molecular characterization; read and evaluate scientific literature; and receive training in scientific communication through written and oral exercises that incorporate social media elements. In the first three semesters, the …


Embl2checklists: A Python Package To Facilitate The User-Friendly Submission Of Plant And Fungal Dna Barcoding Sequences To Ena, Michael Gruenstaeudl, Yannick Hartmaring Jan 2019

Embl2checklists: A Python Package To Facilitate The User-Friendly Submission Of Plant And Fungal Dna Barcoding Sequences To Ena, Michael Gruenstaeudl, Yannick Hartmaring

Biological Sciences Faculty Publications

Background The submission of DNA sequences to public sequence databases is an essential, but insufficiently automated step in the process of generating and disseminating novel DNA sequence data. Despite the centrality of database submissions to biological research, the range of available software tools that facilitate the preparation of sequence data for database submissions is low, especially for sequences generated via plant and fungal DNA barcoding. Current submission procedures can be complex and prohibitively time expensive for any but a small number of input sequences. A user-friendly software tool is needed that streamlines the file preparation for database submissions of DNA …