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Full-Text Articles in Biology
A Bacterial Microbiome Analysis Of Solarized Ginseng Garden Soils, Anka Colo
A Bacterial Microbiome Analysis Of Solarized Ginseng Garden Soils, Anka Colo
Undergraduate Student Research Internships Conference
American ginseng (Panax quinquefolius) is a highly valued perennial crop grown for its roots during a four-year cultivation cycle. American ginseng is subject to ginseng replant disease (GRD) in which severe root rot develops in newly planted ginseng grown in a former ginseng garden. A common strategy to mitigating GRD is not available and techniques such as fumigation, fungicides, and biocontrol are ineffective, banned, or are slowly being phased out. Alternatively, soil solarization is a pre-plant technique used to treat soil to reduce disease inoculum and alter soil microbiomes. In summer 2019, a six-week soil solarization experiment was …
Optimization Of Dna Extraction From Difficult Sandy-Loam Soils, Anka Colo
Optimization Of Dna Extraction From Difficult Sandy-Loam Soils, Anka Colo
Undergraduate Student Research Internships Conference
Ginseng is a high value perennial crop grown for its roots. A four-year cultivation cycle is required to obtain marketable ginseng roots, during which a condition called ginseng replant disease (GRD) develops. GRD is characterized by a severe root rot disease, and prevents the growth of newly planted ginseng in a former ginseng garden1. Microbiome analysis of ginseng garden soil will help our understanding of the formation of GRD and its severity. However, extracting DNA from the sandy-loam soils required for ginseng cultivation is difficult. Commercial kits are either too expensive or not applicable to the large volumes of soil …
The Analysis Of Ginsenosides In Ginseng Garden Soil, Karina Marie Kaberi
The Analysis Of Ginsenosides In Ginseng Garden Soil, Karina Marie Kaberi
Electronic Thesis and Dissertation Repository
American ginseng (Panax quinquefolius L.) produces natural products called ginsenosides. The biggest challenge Ontario commercial ginseng farmers face is ginseng replant disease. To understand the function of ginseng root exudates, ginsenoside accumulation and persistence over time were investigated. Currently, no reliable ginsenoside specific extraction method, characterizing the changes in soil chemistry exists. Ginsenoside extraction protocol optimization was required to determine how ginsenoside composition changed over time. Overall, protocol optimization resulted in a 30% increase in yield of ginsenosides compared to previous extraction protocols. In the ginseng gardens, ginsenoside accumulation occurs slowly and did not reach significantly measurable amounts until the …