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Full-Text Articles in Biology
Metacaspase Gene Function In The Mushroom Fungus Schizophyllum Commune, Matthew P. Hanley
Metacaspase Gene Function In The Mushroom Fungus Schizophyllum Commune, Matthew P. Hanley
Honors Theses
The overall goal of this project was to investigate the biological role of a putative metacaspase gene present in the mushroom fungus Schizophyllum commune. For this study, we have utilized a strain of S. commune that is unable to integrate DNA via the non-homologous end joining pathway. This forces transforming DNA to integrate homologously, as is required for the purposes of gene knockout. The gene Scp1 encodes a likely member of the metacaspase protein family, which are suspected to have activity similar to caspases, the latter crucial to programmed cell death. A knockout construct containing a non-functional version of Scp1 …
Development Of A Robust Genetic Test For Hyperkalemic Periodic Paralysis (Hypp) In Quarter Horses, Samantha Traver
Development Of A Robust Genetic Test For Hyperkalemic Periodic Paralysis (Hypp) In Quarter Horses, Samantha Traver
Honors Theses
A single nucleotide substitution in a region of the skeletal muscle sodium channel gene (SCN4A) is known to cause an equine genetic disorder known as Hyperkalemic Periodic Paralysis (HYPP). The clinical effects of this disorder range from little or no symptoms to frequent episodes of muscle tremors, weakness, and/or complete collapse. Oligonucleotide primer pairs were designed for both the wild type and mutant alleles of the SCN4A gene for use in Amplification Refractory Mutation System-PCR (ARMS-PCR). These primers were tested with genomic DNA isolated from whole blood, saliva swabs, and hair of individual horses. It was determined that horse hair …
Effects Of Arginine On The Kinetics Of Bovine Insulin Aggregation Studied By Dynamic Light Scattering, Michael M. Varughese
Effects Of Arginine On The Kinetics Of Bovine Insulin Aggregation Studied By Dynamic Light Scattering, Michael M. Varughese
Honors Theses
In the fields of protein science and medicine, understanding the kinetics of protein aggregation are significant in the research and treatment of certain amyloid diseases such as Alzheimer’s. Previous studies have suggested that arginine can increase the solubility of certain proteins, suppress protein aggregation, and assist in the refolding of aggregated proteins; however, the molecular mechanisms by which arginine can influence protein aggregation are still unclear. Bovine insulin was employed as a model system for further understanding the effects of arginine on protein aggregation. Using Dynamic Light Scattering (DLS), we studied the concentration-dependent and temperature-dependent suppression of aggregation in insulin …
The Impact Of Tricaine Methanesulfonate, 2-Phenoxyethanol, And Carvone-Methyl Salicylate On The Innate Immune Response Of Zebrafish (Danio Rerio), Charles R. Wulff
The Impact Of Tricaine Methanesulfonate, 2-Phenoxyethanol, And Carvone-Methyl Salicylate On The Innate Immune Response Of Zebrafish (Danio Rerio), Charles R. Wulff
Honors Theses
Anesthesia plays a vital role in the maintenance of aquaculture species, where it is used to minimize stress during complex handling tasks such as transport, assessment, and harvesting. However, anesthetics have been shown to suppress the innate immune response, which could impact immunity and increase risk of infection. Tricaine methanesulfonate (MS-222) and 2-Phenoxyethanol (2-PE) represent two of the most commonly used anesthetics in aquaculture, with R-(+)-carvone, in the form of carvone-methyl salicylate (CMS) has recently been proposed as an alternative anesthetic for food fish. These three anesthetics were used to assess the influence of anesthetics on the immune system of …