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Aspects Of Cardiovascular Oxygen Transport In Vertebrates, Michael Scott Hedrick
Aspects Of Cardiovascular Oxygen Transport In Vertebrates, Michael Scott Hedrick
Dissertations and Theses
The hematological and rheological characteristics of blood from a number of vertebrates was compared to assess possible species differences in blood viscosity that may influence cardiovascular oxygen transport. Nucleated red blood cells (RBCs) were more viscous (measured by cone-plate viscometry) in comparison with enucleate (mammalian) RBCs at hematocrits greater than 40% when measured at equivalent temperatures. The lower viscosity of enucleate RBCs is attributed to an enhanced deformability of enucleate cells in comparison to nucleated cells.
Partial Purification And Characterization Of F₄₂₀-Dependent Nadp Reductase From Methanobrevibacter Smithii Strain De1, Scott D. Sheridan
Partial Purification And Characterization Of F₄₂₀-Dependent Nadp Reductase From Methanobrevibacter Smithii Strain De1, Scott D. Sheridan
Dissertations and Theses
The F420-dependent NADP reductase of Methanobrevibacter smithii has been partially purified employing a combination of affinity chromatography with Blue Sepharose (Cl-6B) and molecular sieve chromatography with Sephacryl S-200, The enzyme, which requires reduced F420 as an electron donor, has been purified over 145 fold with a recovery of 6%. A molecular weight of 120,00 for the native enzyme was determined by Sephacryl S-200 chromatography. A subunit molecular weight of 28,200 was determined by SDS-PAGE, indicating that the native enzyme is a tetramer. The optimal temperature for enzymatic activity was found to be 45°C, with a pH optimum …