Biology Commons^™

A Novel On‐Chip Method For Differential Extraction Of Sperm In Forensic Cases, Fatih Inci, Mehmet O. Ozen, Yeseren Saylan, Morteza Miansari, Duygu Cimen, Raghu Dhara, Thiruppathiraja Chinnasamy, Mehmet Yuksekkaya, Chiara Filippini, Deepan Kishore Kumar, Semih Calamak, Yusuf Yesil, Naside Gozde Durmus, George Duncan, Leonard Klevan, Utkan Demirci

Biology Faculty Articles

One out of every six American women has been the victim of a sexual assault in their lifetime. However, the DNA casework backlog continues to increase outpacing the nation's capacity since DNA evidence processing in sexual assault casework remains a bottleneck due to laborious and time‐consuming differential extraction of victim's and perpetrator's cells. Additionally, a significant amount (60–90%) of male DNA evidence may be lost with existing procedures. Here, a microfluidic method is developed that selectively captures sperm using a unique oligosaccharide sequence (Sialyl‐LewisX), a major carbohydrate ligand for sperm‐egg binding. This method is validated with forensic mock samples dating …