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Full-Text Articles in Biology
Stellaris Fishing 20131125mon Part 2 Of 2, George Mcnamara
Stellaris Fishing 20131125mon Part 2 Of 2, George Mcnamara
George McNamara
Stellaris FISH dataset using three FISH probe sets.
Slides courtesy of Biosearch Technologies,
https://www.biosearchtech.com/store/product.aspx?catid=224,318,324
see http://stellarisfish.smugmug.com/ for online gallery by Biosearch.
This experiment was to evaluate the crosstalk between the Biosearch fluorophores:
Quasar 570
CAL Fluor Red 610 (CFR 610)
Quasar 670
DAPI (DNA counterstain)
Autofluorescence (green, but sometimes showing up in other channels).
and our lab's Leica DMI6000 fluorescence microscope with Leica filter sets:
DAPI
GFP (L5)
Cy3 (N3)
Texas Red (TxRed2)
Cy5 (Y5)
I also acquired green channel and red channel with exciter filters in our ASI excitation wheel:
GFP + 492 exciter
Texas Red (TxRed2) + 572 …
Flash4 Dark Reference Images, George Mcnamara
Flash4 Dark Reference Images, George Mcnamara
George McNamara
Hamamatsu FLASH4.0 dark reference images, acquired with 10 second exposure times, no light to camera. Camera offset (set by Hamamatsu( is ~100 (the average intensity of the first image is always ~1 intensity level higher - an odd feature, but trivial in practice for a 16-bit camera).
George McNamara, Ph.D.
Single Cells Analyst at L.J.N. Cooper Lab
University of Texas M.D. Anderson Cancer Center
Video Codec Performance (Excel Spreadsheet), George Mcnamara
Video Codec Performance (Excel Spreadsheet), George Mcnamara
George McNamara
Video codec performance (Excel spreadsheet). Movie was made in 2005-2006 when I worked at City of Hope National Medical Center. VTLF refers to Video Timelapse Light Facility. Videos were outputted from MetaMorph as AVI files. Personally, I always recommend uncompressed video files fro scientific uses. I also encourage posting the original scientific data format (ex. .lsm, .zvi, .lif, .stk).
Pubspectra Tattletales, George Mcnamara
Pubspectra Tattletales, George Mcnamara
George McNamara
Tattletales for Multiplex Fluorescent Reporters in Single Cells for Metabolomics
George McNamara
As of April 2013: L.J.N. Cooper & D.A. Lee Cellular Immunotherapy Lab, University of Texas M.D. Anderson Cancer Center, Houston, TX
Email: gtmcnamara@mdanderson.org, geomcnamara@earthlink.net
Tattletales is my concept for spatial multiplexing many fluorescent protein (FP) biosensors in the same live cell. For example, there are excellent FP biosensors to Ca++ ions, pH, glucose, ribose, glutamine, glutamate, ATP, redox, ROS, pyruvate, cAMP, cGMP, IP3, PI(3,4,5)P3, cell cycle indicators (Fucci2), PKA, PKC, photsphatases, caspase(s) [1, 2]. However, these are typically used one biosensor per experiment, due in part to flooding …
Characterization Of Genes Required For Preimplantation Embryo Development, Marc P. Maserati Jr
Characterization Of Genes Required For Preimplantation Embryo Development, Marc P. Maserati Jr
Marc P Maserati Jr
Preimplantation embryo development in the mouse is a time of rapid cellular morphological and molecular changes leading to embryo implantation for the generation of offspring. The Mager lab studies these events occuring between fertilization and implantation in order to better understand the initial events which set the stage for all future aspects of development. The result of this research impacts many scientific disciplines including in-vitro based means of embryo culture, establishment of epigenetic marks, differentiation and cellular reprogramming and can be used in translational research for the improvement of in-vitro culture techniques and develop novel therapies such as cell replacement …
Hydraphiles: A Rigorously Studied Class Of Synthetic Channel Compounds With In Vivo Activity, Saeedeh Negin, Bryan A. Smith, Alexandra Unger, W. Matthew Leevy, George W. Gokel
Hydraphiles: A Rigorously Studied Class Of Synthetic Channel Compounds With In Vivo Activity, Saeedeh Negin, Bryan A. Smith, Alexandra Unger, W. Matthew Leevy, George W. Gokel
George Gokel
No abstract provided.