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Boesenbergia rotunda Medicinal plant Micropropagation Zingiberaceae 1 naphthylacetic acid 6 n benzyladenine gelrite sucrose acclimatization article bud concentration (parameters) culture technique inoculation Murashige and Skoog medium nonhuman rhizome rooting shoot
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Rapid Micropropagation Of Boesenbergia Rotunda (L.) Mansf. Kulturpfl. (A Valuable Medicinal Plant) From Shoot Bud Explants, Norzulaani Khalid
Rapid Micropropagation Of Boesenbergia Rotunda (L.) Mansf. Kulturpfl. (A Valuable Medicinal Plant) From Shoot Bud Explants, Norzulaani Khalid
Norzulaani Khalid
A successful protocol was developed for mass propagation of Boesenbergia rotunda (L.) Mansf. Kulturpfl., an important medicinal plant. Numerous shoots were induced from young shoot bud of B. rotunda mature rhizome on Murashige and Skoog (1962) medium supplemented with 30.0 g/l sucrose, 2.0 g/l gelrite, different concentrations of 6-benzylaminopurine (BAP) and α-naphtaleneacetic acid (NAA). Plant medium supplemented with different concentrations of BAP alone or with NAA produced varying degree of multiple shoots. A supplementation of 2.0 mg/l BAP and 0.5 mg/l NAA gave the best result. Ninety percent of the explants induced multiple shoots within 10 to 14 days of …