Open Access. Powered by Scholars. Published by Universities.®

Bioinformatics Commons™

Open Access. Powered by Scholars. Published by Universities.®

Selected Works

Gene expression

Articles 1 - 4 of 4

Full-Text Articles in Bioinformatics

A Gene-Based Association Method For Mapping Traits Using Reference Transcriptome Data, Eric R. Gamazon, Heather Wheeler, Kaanan P. Shah, Sahar V. Mozaffari, Keston Aquino-Michaels, Robert J. Carroll, Anne E. Eyler, Joshua C. Denny, Dan L. Nicolae, Nancy J. Cox, Hae Kyung Im Aug 2016

A Gene-Based Association Method For Mapping Traits Using Reference Transcriptome Data, Eric R. Gamazon, Heather Wheeler, Kaanan P. Shah, Sahar V. Mozaffari, Keston Aquino-Michaels, Robert J. Carroll, Anne E. Eyler, Joshua C. Denny, Dan L. Nicolae, Nancy J. Cox, Hae Kyung Im

Heather Wheeler

Genome-wide association studies (GWAS) have identified thousands of variants robustly associated with complex traits. However, the biological mechanisms underlying these associations are, in general, not well understood. We propose a gene-based association method called PrediXcan that directly tests the molecular mechanisms through which genetic variation affects phenotype. The approach estimates the component of gene expression determined by an individual’s genetic profile and correlates ‘imputed’ gene expression with the phenotype under investigation to identify genes involved in the etiology of the phenotype. Genetically regulated gene expression is estimated using whole-genome tissue-dependent prediction models trained with reference transcriptome data sets. PrediXcan enjoys …

Go to article

Distinct Cardiac Responses To Heat Stress Between Two Broiler Lines Identified By Transcriptome Analysis, Jibin Zhang, Carl J. Schmidt, Susan J. Lamont Jan 2016

Distinct Cardiac Responses To Heat Stress Between Two Broiler Lines Identified By Transcriptome Analysis, Jibin Zhang, Carl J. Schmidt, Susan J. Lamont

Jibin Zhang

Prolonged exposure to high temperature can significantly increase metabolism, depress appetite, reduce meat production and raise mortality in broilers. Because the cardiovascular system plays an important role to dissipate heat and transport oxygen, strong cardiac function is indispensable for birds to survive under heat stress. However, due to small hearts relative to body weight, modern broilers have high risk of heart failure at high temperature.
In this study, a Ross 708 line (modern broilers) and a Heritage broiler line (relaxed selection since the 1950s) were subjected to heat stress at 35℃  for 7 hours/day from 21 to 42 days posthatch …

Go to article

Jibin Zhang's Ppt For Midwest Meeting.Pdf, Jibin Zhang, Yeunsu Suh, Young Min Choi, Michael E. Davis, Kichoon Lee Mar 2014

Jibin Zhang's Ppt For Midwest Meeting.Pdf, Jibin Zhang, Yeunsu Suh, Young Min Choi, Michael E. Davis, Kichoon Lee

Jibin Zhang

Increasing numbers of fat cells in adipose tissue are attributed to proliferation of preadipocytes or immature adipocytes in the early stages as well as adipogenic differentiation in the later stages of adipogenesis. Although both events are involved in the increase in fat cell numbers, they are contrary to each other in that the former requires cell cycle activity whereas the latter requires cell cycle withdrawal. Therefore, appropriate regulation of cell cycle inhibition is critical to adipogenesis.
To explore the important cell cycle inhibitors and study their expression in adipogenesis, a strategy was adopted that combined the Gene Expression Omnibus (GEO) …

Go to article

Description And Annotation Of Biomedical Data Sets, Jen Ferguson Feb 2012

Description And Annotation Of Biomedical Data Sets, Jen Ferguson

Jen Ferguson

Deposition of biomedical data sets is on the rise as more scientists submit experimental data to accompany their publications. Scientists are also increasingly reusing these publicly available data sets in their own work. Despite these developments, lack of both context and metadata can create barriers to understanding and repurposing these data sets. Researchers from the Bioinformatics Core Group in the Harvard School of Public Health attempted to address this issue by assembling a team of data curators who used the open source software suite ISA tools to annotate and contextualize microarray data sets. This paper describes the workflow and software …

Go to article