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Full-Text Articles in Biodiversity
The Significant Surface-Water Connectivity Of “Geographically Isolated Wetlands", Aram J.K. Calhoun, David M. Mushet, Laurie C. Alexander, Edward S. Dekeyser, Laurie Fowler, Megan W. Lang, Mark C. Rains, Stephen C. Richter, Susan C. Walls
The Significant Surface-Water Connectivity Of “Geographically Isolated Wetlands", Aram J.K. Calhoun, David M. Mushet, Laurie C. Alexander, Edward S. Dekeyser, Laurie Fowler, Megan W. Lang, Mark C. Rains, Stephen C. Richter, Susan C. Walls
EKU Faculty and Staff Scholarship
We evaluated the current literature, coupled with our collective research expertise, on surface-water connectivity of wetlands considered to be “geographically isolated” (sensu Tiner Wetlands 23:494–516, 2003a) to critically assess the scientific foundation of grouping wetlands based on the singular condition of being surrounded by uplands. The most recent research on wetlands considered to be “geographically isolated” shows the difficulties in grouping an ecological resource that does not reliably indicate lack of surface water connectivity in order to meet legal, regulatory, or scientific needs. Additionally, the practice of identifying “geographically isolated wetlands” based on distance from a stream can …
Sample Collection And Dna Extraction Methods For Environmental Dna Metabarcoding In Headwater Streams, Michael Triston Mullins
Sample Collection And Dna Extraction Methods For Environmental Dna Metabarcoding In Headwater Streams, Michael Triston Mullins
Online Theses and Dissertations
DNA is found to be free and ubiquitous in the environment where it is no longer associated with the source organism, and is also known as environmental-DNA (eDNA). Methods optimized for specific environments may be able supplement insight to local taxa richness. With the advent of high throughput sequencing and the proliferation of sequence data in public repositories, insights to the biodiversity of communities at the molecular level have been possible. Thus, this study compared commonly used DNA capture (water precipitation and filtration) and extraction (MoBio's PowerWater, Qiagen's DNeasy Blood and Tissue Kit, and a CTAB protocol) methods in their …