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Ohio Northern University

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Genetic Variation In The Ohio Population Of Tonguetied Minnow (Exoglossum Laurae), Emmily Moses, Sarah Armstrong, Madeline Williams Apr 2022

Genetic Variation In The Ohio Population Of Tonguetied Minnow (Exoglossum Laurae), Emmily Moses, Sarah Armstrong, Madeline Williams

ONU Student Research Colloquium

A singular population of tonguetied minnow (Exoglossum laurae) inhabits a ~50-km segment of the Mad River of western Ohio. Tonguetied minnow is Endangered in Ohio as a consequence of its small geographic range and low abundance. For this study, the polymerase chain reaction (PCR) was used amplify five genetic loci from 35 tonguetied minnow captured from the Mad River. Loci included two mitochondrial genes, the NADH-dehydrogenase 2 (ND2) and the control region (D-loop). From the nuclear genome, myosin heavy chain 6 (myh6) and β-actin (β-act) introns were amplified, as well as a portion of the major histocompatibility (Mhc-IIB) …