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Full-Text Articles in Other Biochemistry, Biophysics, and Structural Biology
Multiple Factors Independently Regulate Hila And Invasion Gene Expression In Salmonella Enterica Serovar Typhimurium, Robin L. Lucas, C. Phoebe Lostroh, Concetta C. Dirusso, Michael P. Spector, Barry L. Wanner, Catherine A. Lee
Multiple Factors Independently Regulate Hila And Invasion Gene Expression In Salmonella Enterica Serovar Typhimurium, Robin L. Lucas, C. Phoebe Lostroh, Concetta C. Dirusso, Michael P. Spector, Barry L. Wanner, Catherine A. Lee
Department of Biochemistry: Faculty Publications
HilA activates the expression of Salmonella enterica serovar Typhimurium invasion genes. To learn more
about regulation of hilA, we isolated Tn5 mutants exhibiting reduced hilA and/or invasion gene expression. In
addition to expected mutations, we identified Tn5 insertions in pstS, fadD, flhD, flhC, and fliA. Analysis of the
pstS mutant indicates that hilA and invasion genes are repressed by the response regulator PhoB in the absence
of the Pst high-affinity inorganic phosphate uptake system. This system is required for negative control of the
PhoR-PhoB two-component regulatory system, suggesting that hilA expression may …
Multiple Factors Independently Regulate Hila And Invasion Gene Expression In Salmonella Enterica Serovar Typhimurium, Robin L. Lucas, C. Phoebe Lostroh, Concetta C. Dirusso, Michael P. Spector, Barry L. Wanner, Catherine A. Lee
Multiple Factors Independently Regulate Hila And Invasion Gene Expression In Salmonella Enterica Serovar Typhimurium, Robin L. Lucas, C. Phoebe Lostroh, Concetta C. Dirusso, Michael P. Spector, Barry L. Wanner, Catherine A. Lee
Department of Biochemistry: Faculty Publications
HilA activates the expression of Salmonella enterica serovar Typhimurium invasion genes. To learn more
about regulation of hilA, we isolated Tn5 mutants exhibiting reduced hilA and/or invasion gene expression. In
addition to expected mutations, we identified Tn5 insertions in pstS, fadD, flhD, flhC, and fliA. Analysis of the
pstS mutant indicates that hilA and invasion genes are repressed by the response regulator PhoB in the absence
of the Pst high-affinity inorganic phosphate uptake system. This system is required for negative control of the
PhoR-PhoB two-component regulatory system, suggesting that hilA expression may …
Two-Step Processing Of Human Frataxin By Mitochondrial Processing Peptidase, Patrizia Cavadini, Jiri Adamec, Franco Taroni, Oleksandr Gakh, Grazia Isaya
Two-Step Processing Of Human Frataxin By Mitochondrial Processing Peptidase, Patrizia Cavadini, Jiri Adamec, Franco Taroni, Oleksandr Gakh, Grazia Isaya
Department of Biochemistry: Faculty Publications
We showed previously that maturation of the human
frataxin precursor (p-fxn) involves two cleavages by the
mitochondrial processing peptidase (MPP). This observation
was not confirmed by another group, however,
who reported only one cleavage. Here, we demonstrate
conclusively that MPP cleaves p-fxn in two sequential
steps, yielding a 18,826-Da intermediate (i-fxn) and a
17,255-Da mature (m-fxn) form, the latter corresponding
to endogenous frataxin in human tissues. The two cleavages
occur between residues 41–42 and 55–56, and both
match the MPP consensus sequence RX ↓ (X/S). Recombinant
rat and yeast MPP catalyze the pài step 4 and 40 …