Other Biochemistry, Biophysics, and Structural Biology Commons^™

Development Of An Electrochemical Technique For Oxidative Surface Mapping To Investigate Solution-Phase Protein Dynamics With High Performance Mass Spectrometry And Advanced Informatics, Carlee Suzanne Patterson Mcclintock

Doctoral Dissertations

Oxidative protein surface mapping has gained popularity over recent years within the mass spectrometry (MS) community for gleaning information about the solvent accessibility of folded protein structures. The hydroxyl radical targets a wide breadth of reactive amino acids with a stable mass tag that withstands subsequent MS analysis. A variety of techniques exist for generating hydroxyl radicals, with most requiring sources of radiation or caustic oxidizing reagents. The purpose of this research was to evaluate the novel use of electrochemistry for accomplishing a comparable probe of protein structure with a more accessible tool. Two different working electrode types were tested …