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Threonine Deficiency In Hydrolysates Of Zein Prepared By Autoclaving, Raymond Borchers, John R. Totter, Clarence P. Berg
Threonine Deficiency In Hydrolysates Of Zein Prepared By Autoclaving, Raymond Borchers, John R. Totter, Clarence P. Berg
Department of Biochemistry: Faculty Publications
In a previous paper (Borchers and Berg, 1942) we showed that
autoclaving zein with sulfuric acid longer than necessary for complete
hydrolysis causes destruction and racemization; either of
these might account for the failure of such a hydrolysate to
promote growth in young rats when substituted for a hvdrolysate,
prepared by refiuxing, in a diet which produced moderate growth.
It seemed to us that this deterioration in the dietary protein might
well be the result primarily of essential amino acid deficiencies
which could be detected and overcome by appropriate supplementation.
Because threonine is known to be present in zein in …
Tryptophane Metabolism X. The Effect Of Feeding 1(-)-, Dl-, And D(+)-Tryptophane, D(-)And Dl-(Β-3-Indolelactic Acid,(Β-3-Indolepyruvic Acid, And L(-)Kynurenine Upon The Storage Of Liver Glycogen And The Urinary Output Of Kynurenic Acid, Kynurenine, And Total Acetone Bodies, Raymond Borchers, Clarence P. Berg, Newton E. Whitman
Tryptophane Metabolism X. The Effect Of Feeding 1(-)-, Dl-, And D(+)-Tryptophane, D(-)And Dl-(Β-3-Indolelactic Acid,(Β-3-Indolepyruvic Acid, And L(-)Kynurenine Upon The Storage Of Liver Glycogen And The Urinary Output Of Kynurenic Acid, Kynurenine, And Total Acetone Bodies, Raymond Borchers, Clarence P. Berg, Newton E. Whitman
Department of Biochemistry: Faculty Publications
The amount of tryptophane required for maintenance and growth is small (2). When an excess is supplied to some animals, kynurenic acid is excreted (3) and, under certain conditions, kynurenine also (4). Although these are quantitatively the most important of the known metabolic products of tryptophane, the amounts excreted in extensive tests in the dog and rabbit have usually accounted for less than half, more often for less than a third, of the tryptophane administered (4-6).
The Effect Of Conditions Of Hydrolysis And Of Prolonged Heating Upon The Optical Rotation Of Sulfuric Acid Hydrolysates Of Zein, Raymond Borchers, Clarence P. Berg
The Effect Of Conditions Of Hydrolysis And Of Prolonged Heating Upon The Optical Rotation Of Sulfuric Acid Hydrolysates Of Zein, Raymond Borchers, Clarence P. Berg
Department of Biochemistry: Faculty Publications
Some time ago we observed that a sulfuric acid hydrolysate of zein prepared in an autoclave at 165˚ did not support growth in young rats when it was supplemented with certain amino acids, even though the same amino acids, added to a hydrolysate prepared by refluxing, permitted moderate growth. Since the optical rotation of the autoclaved hydrolysate was lower than that of the refluxed, either destruction or racemization of essential constituents, or both, might have occurred. Loss of amino nitrogen and production of extra ammonia upon prolonged autoclaving of proteins with acids have been noted by Van Slyke (1912) …