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Biochemistry, Biophysics, and Structural Biology Commons™
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- SSA4 (3)
- MRNA export (2)
- Biochemistry, mRNA export, bacterial induction, transcription, translation, purification (1)
- CRISPR (1)
- Chaperones (1)
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- Condition testing (1)
- GFP (1)
- Gene expression (1)
- Heat shock (1)
- MRNA (1)
- Mex67 (1)
- Molecular cloning (1)
- Multicopy Suppression Screen (1)
- Nuclear pore complex (1)
- QPCR (1)
- S. cerevisiae (1)
- Saccharomyces cerevisiae (1)
- Saccharomyces cerevisiae , split fluorescent microscopy, nuclear pore complex, LINC complex, Venus split - GFP, mRNA export, mechanical stress, nuclear envelope, Mps3, Ndj1 (1)
- Selective export (1)
- Selective mRNA export (1)
- Yeast Three-Hybrid (1)
Articles 1 - 5 of 5
Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Analysis Of Ssa4 Reporter Expression By Q-Pcr, Susveen Sharanshi, Rebecca Adams
Analysis Of Ssa4 Reporter Expression By Q-Pcr, Susveen Sharanshi, Rebecca Adams
Belmont University Research Symposium (BURS)
The synthesis of genome-encoded proteins via mRNA translation is integral to cell survival. In eukaryotes, such as S. cerevisiae, the mRNA that is produced in the nucleus must be exported to the cytoplasm for translation to occur, and this process is highly regulated. Specifically, the export of mRNA occurs via travel through nuclear pore complexes (NPCs), which are selective doorways embedded in the nuclear envelope. During cellular stress, such as heat shock, the cell needs to regulate gene expression to permit survival, and mRNA export is one step at which this occurs. At these high temperatures, a cell’s proteins …
Establishing A Biochemical System For The Purification And Atpase Activity Of Gst-Dbp5, Sarah Utley, Rachel E. Rigsby Phd, Rebecca L. Adams Phd
Establishing A Biochemical System For The Purification And Atpase Activity Of Gst-Dbp5, Sarah Utley, Rachel E. Rigsby Phd, Rebecca L. Adams Phd
Science University Research Symposium (SURS)
The export of mRNA out of the nucleus is a crucial step for eukaryotic gene expression. The export of mRNA transcripts is aided by Mex67, which allows export through the nuclear pore complex doorways in the nuclear envelope. Once out of the nucleus, a protein known as Dbp5, bound to ATP, Gle1, and Nup42 aids in the directionality of mRNA export by helping remove Mex67 from the mRNA strand. Following interaction with RNA, Dbp5 then hydrolyzes ATP so that it unbinds the mRNA, allowing for enzyme recycling. The goal of this study is to explore the ATPase activity of Saccharomyces …
Testing Ssa4:Ade3 Reporters For Mcs Screening, Karah Edmonds, Rebecca Adams
Testing Ssa4:Ade3 Reporters For Mcs Screening, Karah Edmonds, Rebecca Adams
Science University Research Symposium (SURS)
In eukaryotic cells, after transcription, mRNA is escorted from the nucleus to the cytoplasm to be translated. This process, called mRNA export, is essential for gene expression. However, when the cell exists in stressful conditions, mRNA export becomes regulated, and only select transcripts, including the stress-responsive SSA4 mRNA, can be exported. This project aims to uncover the mechanism of selective SSA4 mRNA export by generating a reporter that enables phenotypically visible expression under stressful conditions. Specifically, the ADE3 ORF was placed under the regulatory sequence of SSA4, which was anticipated to induce a red color for colonies only following stress. …
Detecting Linc Complex Mps3 And Nuclear Pore Complex Ndj1 Protein Interactions On Yeast Nuclear Membrane S Through Fluorescence Microscopy, Dean Boecher, Rebecca Adams
Detecting Linc Complex Mps3 And Nuclear Pore Complex Ndj1 Protein Interactions On Yeast Nuclear Membrane S Through Fluorescence Microscopy, Dean Boecher, Rebecca Adams
Science University Research Symposium (SURS)
Though cancer cells have been shown to have abnormal nuclear morphologies and responses to mechanical forces, the mechanisms of how mechanical stress is translated into cellular action and structural reorganization within the nuclear envelope are largely unexplored. The Linker of Nucleoskeleton and Cytoskeleton (LINC) complex is a transmembrane protein complex that connects the actin cytoskeleton to the lamin nucleoskeleton, enabling mechanical forces to be translated between the cytoplasm and the nucleus. In cells exposed to physical stress, nuclear pore complexes (NPCs) –which control the exchange of biochemical signals and macromolecules in and out of the nucleus through mRNA export –have …
Analyzing Mex67 Interaction With The Ssa4 Transcript For Selective Export, Gretchen E. Stalnaker, Rebecca Adams
Analyzing Mex67 Interaction With The Ssa4 Transcript For Selective Export, Gretchen E. Stalnaker, Rebecca Adams
Science University Research Symposium (SURS)
In eukaryotic cells, such as the budding yeast Saccharomyces cerevisiae, mRNA export is the essential process in which mature mRNA is transported from its site of production, the nucleus, through the nuclear pore complex (NPC) to the cytoplasm, where it can then be translated into protein. This is accomplished when a transcript interacts with the mRNA export protein Mex67, which shuttles the message across the NPC. When the cell is under conditions of stress, such as heat shock, most mRNA export is prohibited, allowing for selective gene expression that prioritizes cell recovery. Specifically, SSA4, which encodes a protein that …