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Articles 1 - 11 of 11

Full-Text Articles in Biochemistry, Biophysics, and Structural Biology

Collaborative Roles For C-Jun N-Terminal Kinase, C-Jun, Serum Response Factor, And Sp1 In Calcium-Regulated Myocardial Gene Expression, Patrick M. Mcdonough, Deanna S. Hanford, Amy B. Sprenkle, Noel R. Mellon, Christopher C. Glembotski Sep 1997

Collaborative Roles For C-Jun N-Terminal Kinase, C-Jun, Serum Response Factor, And Sp1 In Calcium-Regulated Myocardial Gene Expression, Patrick M. Mcdonough, Deanna S. Hanford, Amy B. Sprenkle, Noel R. Mellon, Christopher C. Glembotski

Amy Sprenkle

Electrical stimulation of contractions (pacing) of primary neonatal rat ventricular myocytes increases intracellular calcium and activates a hypertrophic growth program that includes expression of the cardiac-specific gene, atrial natriuretic factor (ANF). To investigate the mechanism whereby pacing increases ANF, pacing was tested for its ability to regulate mitogen-activated protein kinase family members, ANF promoter activity, and the trans-activation domain of the transcription factor, Sp1. Pacing and the calcium channel agonist BAYK 8644 activated c-Jun N-terminal kinase (JNK) but not extracellular signal-regulated kinase. Pacing stimulated ANF-promoter activity approximately 10-fold. Furthermore, transfection with an expression vector for c-Jun, a substrate for JNK, …


Ms2 Coat Protein Mutants Which Bind Qβ Rna, Marc Spingola, David S. Peabody Jul 1997

Ms2 Coat Protein Mutants Which Bind Qβ Rna, Marc Spingola, David S. Peabody

Marc Spingola

The coat proteins of the RNA phages MS2 and Qβ are structurally homologous, yet they specifically bind different RNA structures. In an effort to identify the basis of RNA binding specificity we sought to isolate mutants that convert MS2 coat protein to the RNA binding specificity of Qβ. A library of mutations was created which selectively substitutes amino acids within the RNA binding site. Genetic selection for the ability to repress translation from the Qβ translational operator led to the isolation of several MS2 mutants that acquired binding activity for Qβ RNA. Some of these also had reduced abilities to …


Thermodynamics Of Oligoarginines Binding To Rna And Dna., David Mascotti, Timothy Lohman Jun 1997

Thermodynamics Of Oligoarginines Binding To Rna And Dna., David Mascotti, Timothy Lohman

David P. Mascotti

These results suggest that hydrogen bonding of arginine to the phosphate backbone of the nucleic acids contributes to the increased stability of these complexes.


Ac-Mode Atomic Force Microscope Imaging In Air And Solutions With A Thermally Driven Bimetallic Cantilever Probe, Andrew C. Hillier, Allen J. Bard Apr 1997

Ac-Mode Atomic Force Microscope Imaging In Air And Solutions With A Thermally Driven Bimetallic Cantilever Probe, Andrew C. Hillier, Allen J. Bard

Andrew C. Hillier

An ac imaging mode for atomic force microscopy ~AFM! has been developed that employs a thermally driven bimetallic cantilever to sense surface topography. Oscillations are induced in a composite cantilever, comprising a Si3N4 layer and Au overcoat, by local heating with a resistive wire heater placed in close proximity to the cantilever. Cantilever bending occurs upon heating due to the difference in thermal expansion coefficients of the Si3N4 and Au layers. The magnitude of this bending is a function of the heat input, the cantilever geometry, the frequency of the excitation, and the thermal properties of the surrounding medium. A …


The Role Of Molecular Recognition In Activation And Regulation In The Growth Hormone-Prolactin Family Of Hormones And Receptors, Anthony Kossiakoff, Celia Schiffer, Abraham De Vos Dec 1996

The Role Of Molecular Recognition In Activation And Regulation In The Growth Hormone-Prolactin Family Of Hormones And Receptors, Anthony Kossiakoff, Celia Schiffer, Abraham De Vos

Celia A. Schiffer

In: Rick L. Ornstein, Biomacromolecules--from 3-D to applications: Thirty-fourth Hanford Symposium on Health and the Environment, October 23-26, 1995, Pasco, Washington, U.S.A. Battelle Press, 1997, p. 123-131. ISBN 1574770195, 9781574770193.


Involvement Of The Ras-Like Gtpase Rab3d In Rbl-2h3 Mast Cell Exocytosis Following Stimulation Via High Affinity Ige Receptors (Fcεri), Michele Roa, Fabienne Paumet, Joelle Le Mao, Bernard David, Ulrich Blank Dec 1996

Involvement Of The Ras-Like Gtpase Rab3d In Rbl-2h3 Mast Cell Exocytosis Following Stimulation Via High Affinity Ige Receptors (Fcεri), Michele Roa, Fabienne Paumet, Joelle Le Mao, Bernard David, Ulrich Blank

Fabienne Paumet

No abstract provided.


Specificity And Cellular Mechanism Of Lysophosphatidylcholine Stimulation Of Vascular Smooth Muscle Cell Growth., Yuh-Cherng Chai, David Binion, Guy Chisolm Dec 1996

Specificity And Cellular Mechanism Of Lysophosphatidylcholine Stimulation Of Vascular Smooth Muscle Cell Growth., Yuh-Cherng Chai, David Binion, Guy Chisolm

Yuh-Cherng Chai

No abstract provided.


Time-Averaging Crystallographic Refinement, Celia Schiffer Dec 1996

Time-Averaging Crystallographic Refinement, Celia Schiffer

Celia A. Schiffer

In: Wilfred F. van Gunsteren and Paul K. Weiner, Computer Simulation of Biomolecular Systems: Theoretical and Experimental Applications (Volume 3 of Computer Simulation of Biomolecular Systems), Springer, 1997, p. 265-269. ISBN 9072199251, 9789072199256.

Full text of chapter is available through Google Book Search limited preview: Google Book Search description


The Pax-5 Gene Is Alternatively Spliced During B-Cell Development, Patty Zwollo, Hector Arrieta, Kaleo Ede, Karen Molinder, Stephen Desiderio, Roberta Pollock Dec 1996

The Pax-5 Gene Is Alternatively Spliced During B-Cell Development, Patty Zwollo, Hector Arrieta, Kaleo Ede, Karen Molinder, Stephen Desiderio, Roberta Pollock

Roberta Pollock

The transcription factor Pax-5 is expressed during the early stages of B-cell differentiation and influences the expression of several B-cell-specific genes. In addition to the existing isoform (Pax-5, which we have named Pax-5a), we have isolated three new isoforms, Pax-5b, Pax-5d, and Pax-5e, from murine spleen and B-lymphoid cell lines using library screenings and polymerase chain reaction amplification. Isoforms Pax-5b and Pax-5e have spliced out their second exon, resulting in proteins with only a partial DNA-binding domain. Isoforms Pax-5d and Pax-5e have deleted the 3′-region, which encodes the transactivating domain, and replaced it with a novel sequence. The existence of …


Direct Measurement Of Diffuse Double-Layer Forces At The Semiconductor/Electrolyte Interface Using An Atomic Force Microscope, Kai Hu, Fu-Ren F. Fan, Allen J. Bard, Andrew C. Hillier Dec 1996

Direct Measurement Of Diffuse Double-Layer Forces At The Semiconductor/Electrolyte Interface Using An Atomic Force Microscope, Kai Hu, Fu-Ren F. Fan, Allen J. Bard, Andrew C. Hillier

Andrew C. Hillier

The forces between a silica probe and an n-type TiO2 single-crystal electrode were measured using an atomic force microscope in an aqueous electrolyte solution. These interactions were a strong function of the solution pH, the presence of specifically adsorbed anions, and the TiO2 electrode potential. For a series of pH values, a strong electrostatic repulsion was seen at high pH and decreased as the pH was reduced. At pH values below 5.5, the interaction became attractive. A series of force measurements between SiO2 and n-type TiO2 showed a repulsive interaction when TiO2 was held at negative electrode potentials, which transformed …


Genetic Analysis Of Chlorophyll Biosynthesis, David W. Bollivar, Jon Y. Suzuki, Carl E. Bauer Dec 1996

Genetic Analysis Of Chlorophyll Biosynthesis, David W. Bollivar, Jon Y. Suzuki, Carl E. Bauer

David Bollivar

During this decade, there have been major advancements in the understanding of genetic loci involved in synthesis of the family of Mg-tetrapyrroles known as chlorophylls and bacteriochlorophylls. Molecular genetic analysis of Mg-tetrapyrrole biosynthesis was initiated by the performance of detailed sequence and mutational analysis of the photosynthesis gene cluster from Rhodobacter capsulatus. These studies provided the first detailed understanding of genes involved in bacteriochlorophyll a biosynthesis. In the short time since these studies were initiated, most of the chlorophyll biosynthesis genes have been identified by virtue of their ability to complement bacteriochlorophyll a biosynthesis mutants as well as by sequence …