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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Modeling The Tertiary Structure Of A Maize (Zea Mays Ssp. Mays) Non-Symbiotic Hemoglobin, Juán Sáenz-Rivera, Gautam Sarath, Raul Peter-Arredondo
Modeling The Tertiary Structure Of A Maize (Zea Mays Ssp. Mays) Non-Symbiotic Hemoglobin, Juán Sáenz-Rivera, Gautam Sarath, Raul Peter-Arredondo
Gautam Sarath Publications
The tertiary structure of a maize (Zea mays ssp. mays) non-symbiotic hemoglobin (Hbm) was modeled using computer tools and the known tertiary structure of rice Hb1 as a template. This method was tested by predicting the tertiary structure of soybean leghemoglobin a (Lba) using rice Hb1 as a template. The tertiary structures of the predicted and native Lba were similar, indicating that our computer methods could reliably predict the tertiary structures of plant Hbs. We next predicted the tertiary structure of Hbm. Hbm appears to have a long pre-helixA and a large CD-loop. The positions of the distal …
Hospital Universitario, “La Paz”, Madrid, Spain, Michael G. Zeece, Gautam Sarath, John P. Markwell, N. Palmer, J. Steckelberg, J. Schultz
Hospital Universitario, “La Paz”, Madrid, Spain, Michael G. Zeece, Gautam Sarath, John P. Markwell, N. Palmer, J. Steckelberg, J. Schultz
Gautam Sarath Publications
RATIONALE: Proteomic technology has been shown to have distinct advantages for allergen characterization and identification. Therefore, we have used 2-D electrophoresis and protein microarrays to characterize the profile of allergens and assess their level in lentils.
METHODS: Lentil varieties were extracted and centrifuged to obtain 2S, 7S and 11S fractions. The profile of IgE-binding proteins in these fractions was assessed using lentil-allergic sera, by immunoblotting of 2-D electrophoretic separations. The level of IgE-binding was also assessed using protein microarrays. Arrays of covalently attached proteins were first incubated with lentil-allergic sera, followed by incubation with fluorescently- labeled anti-IgE detection antibody. Laser-induced …