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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Shared Thematic Elements In Photochemical Reaction Centers, John H. Golbeck
Shared Thematic Elements In Photochemical Reaction Centers, John H. Golbeck
Department of Biochemistry: Faculty Publications
The structural, functional, and evolutionary relationships between photosystem II and the purple nonsulfur bacterial reaction center have been recognized for several years. These can be classified as "quinone type" (type I) photosystems because the terminal electron acceptor is a mobile quinone molecule. The analogous relationship between photosystem I and the green sulfur bacterial (and heliobacterial) reaction centers has only recently become dear. These can be dcassified as "iron-sulfur type" (type I) photosystems because the terminal electron acceptor consists of one or more bound iron-sulfur clusters. At a fundamental level, the quinone type and ironsulfur type reaction centers share a common …
Mutational Analysis Of The Structure And Biogenesis Of The Photosystem I Reaction Center In The Cyanobacterium Synechocystis Sp. Pcc 6803, Lawrence B. Smart, Patrick V. Warren, John H. Golbeck, Lee Mcintosh
Mutational Analysis Of The Structure And Biogenesis Of The Photosystem I Reaction Center In The Cyanobacterium Synechocystis Sp. Pcc 6803, Lawrence B. Smart, Patrick V. Warren, John H. Golbeck, Lee Mcintosh
Department of Biochemistry: Faculty Publications
We have utilized the unicellular cyanobacterium Synechocystis sp. PCC 6803 to incorporate site-directed amino acid substitutions into the photosystemn I (PSI) reactioncenter protein PsaB. A cysteine residue (position 565 of PsaB) proposed to serve as a ligand to the [4Fe-4S] center Fx was changed to serine, histidine, and aspartate. These three mutants- C565S, C565H, and C565D-all exhibited greatly reduced accumulation of PSI reaction-center proteins and failed to grow autotrophically, indicating that this cysteine most likely does coordinate Fx, which is crucial for PSI biogenesis. Interestingly, the strain C565S accumulated significantly more PSI than the other two cysteine …