Biochemistry, Biophysics, and Structural Biology Commons^™

Monoclonal Antibodies Specific For Mercuric Ions, Dwane E. Wylie, Di Lu, Larry D. Carlson, Randy Carlson, K. Funda Babacan, Sheldon M. Schuster, Fred W. Wagner

Department of Biochemistry: Faculty Publications

Monoclonal antibodies (mAbs) that react with soluble mercuric ions have been produced by i 'ection of BALB/c mice with a hapten-carrier complex designed to maximize exposure of the metal to the immune system. Three hybridomas producing antibodies that reacted with bovine serum albumin (BSA)-glutathione-HgCI, but not with BSAglutathione, were isolated from the spleen of a mouse given multiple iqjections with glutathione-HgCl conjugated to keyhole limpet hemocyanin. Stable subclones were established from two of these antibodies, designated mAb 4A10 and mAb IF10. The binding of both antibodies to immobilized BSAglutathione- HgCl was inhibited by soluble HgCl₂, and dissociation constants …