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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Investigating The Regulatory Circuitry Of Protein Kinases And Proteases In Apoptosis, Stephanie A. Zukowski
Investigating The Regulatory Circuitry Of Protein Kinases And Proteases In Apoptosis, Stephanie A. Zukowski
Electronic Thesis and Dissertation Repository
Apoptosis is a tightly regulated cellular process essential for normal development and tissue homeostasis. Perturbations to apoptotic signaling underscores numerous pathogenic processes emphasizing the importance of apoptotic regulation. During apoptosis, caspases orchestrate cellular degradation through proteolytic cleavage of key structural and enzymatic proteins. In a different manner, protein kinases regulate apoptosis by catalyzing the post-translational phosphorylation of substrate proteins to facilitate either pro- or anti-apoptotic signal transduction pathways. Emerging paradigms have indicated that bidirectional crosstalk between protein kinases and caspases serves to globally fine-tune the equilibrium between signals directing cell survival and cell death. In this regard, identifying points of …
Regulation Of Liver Mitochondrial Metabolism During Hibernation By Post-Translational Modification, Katherine E. Mathers
Regulation Of Liver Mitochondrial Metabolism During Hibernation By Post-Translational Modification, Katherine E. Mathers
Electronic Thesis and Dissertation Repository
Hibernation, characterized by a seasonal reduction in metabolism and body temperature, allows animals to conserve energy when environmental conditions (e.g. temperature, food availability) are unfavourable. During hibernation, small mammals such as the 13-lined ground squirrel (Ictidomys tridecemlineatus) cycle between two distinct metabolic states: torpor, where metabolic rate is suppressed by >95% and body temperature falls to ~5 °C, and interbout euthermia (IBE), where metabolic rate and body temperature rapidly increase and are maintained at euthermic levels several hours. Suppression of metabolism during entrance into torpor is paralleled by rapid suppression of liver mitochondrial metabolism. In my thesis, I …
Quantitative Proteomic Characterization Of Cx-4945, A Clinical Stage Inhibitor Of Protein Kinase Ck2, Adam J. Rabalski
Quantitative Proteomic Characterization Of Cx-4945, A Clinical Stage Inhibitor Of Protein Kinase Ck2, Adam J. Rabalski
Electronic Thesis and Dissertation Repository
Protein phosphorylation is controlled by protein kinases, and represents a critical signaling mechanism involved in the regulation of fundamental biological processes. Furthermore, the aberrant regulation of kinase activity is implicated in diseases such as cancer and has resulted in efforts to target kinases therapeutically. Protein kinase CK2, although frequently considered constitutively active, has emerged as a clinical target on the basis of its altered expression in different types of human cancers and its regulatory participation in multiple biological processes. In fact, CX-4945, a small molecule ATP-competitive inhibitor of CK2 has advanced to clinical trial and has been widely used to …