Biochemistry, Biophysics, and Structural Biology Commons^™

Knockdown Of Limiting-Co2–Induced Gene Hla3 Decreases Hco3− Transport And Photosynthetic Ci Affinity In Chlamydomonas Reinhardtii, Deqiang Duanmu, Amy R. Miller, Kempton M. Horken, Donald P. Weeks, Martin H. Spalding

Department of Biochemistry: Faculty Publications

The CO₂-concentrating mechanism (CCM) of Chlamydomonas reinhardtii and other microalgal species is essential for photosynthetic growth in most natural settings. A great deal has been learned regarding the CCM in cyanobacteria, including identification of inorganic carbon (Ci; CO₂ and HCO₃⁻) transporters; however, specific knowledge of analogous transporters has remained elusive in eukaryotic microalgae such as C. reinhardtii. Here we investigated whether the limiting-CO₂–inducible, putative ABC-type transporter HLA3 might function as a HCO₃⁻ transporter by evaluating the effect of pH on growth, photosynthetic Ci affinity, and [¹⁴C]-Ci uptake in …