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Biochemistry, Biophysics, and Structural Biology Commons™
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University of Nebraska - Lincoln
Department of Biochemistry: Dissertations, Theses, and Student Research
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Articles 1 - 2 of 2
Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Characterization Of The Multifunctional Enzyme Proline Utilization A, Yizi Mao
Characterization Of The Multifunctional Enzyme Proline Utilization A, Yizi Mao
Department of Biochemistry: Dissertations, Theses, and Student Research
Proline is a unique and important amino acid. Proline is a proteogenic amino acid and its metabolism is involved in many critical cellular functions. Therefore, proline metabolism is tightly regulated, and dysfunction of proline metabolism is related to human diseases. The first step of proline oxidization to ∆1-pyrroline-5-carboxylic acid (P5C) is catalyzed by proline dehydrogenase (PRODH). P5C is then non-enzymatically hydrolyzed to glutamate-γ-semialdehyde (GSA), which can be further oxidized to glutamate by P5C dehydrogenase (P5CDH/GSALDH). In Gram-negative bacteria, the PRODH and P5CDH enzymes are expressed as one polypeptide called proline utilization A (PutA). In some Gram-negative bacteria an …
Investigations Of Substrate Channeling In The Proline Oxidative Pathway, Nikhilesh Sanyal
Investigations Of Substrate Channeling In The Proline Oxidative Pathway, Nikhilesh Sanyal
Department of Biochemistry: Dissertations, Theses, and Student Research
In cell metabolism, substrate channeling is a phenomenon where the product of one reaction is transported to a second enzyme active site without equilibrating into bulk solvent. Chapter 1 reviews the rationale and evidence for substrate channeling with the specific example of proline metabolism. Oxidation of proline to glutamate is catalyzed in consecutive reactions by proline dehydrogenase (PRODH) and pyrroline-5-carboxylate dehydrogenase (P5CDH). The intermediate Δ1-pyrroline-5-carboxylate reportedly tends to be labile and inhibitory towards several metabolic pathways.
One of the main objectives of this dissertation was to investigate substrate channeling between independent proline oxidative enzymes from Thermus thermophilus- …