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Biochemistry, Biophysics, and Structural Biology Commons™
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University of Nebraska - Lincoln
Department of Biochemistry: Dissertations, Theses, and Student Research
Articles 1 - 4 of 4
Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Reactive Oxygen Species Homeostasis And Proline Catabolism, Lu Zhang
Reactive Oxygen Species Homeostasis And Proline Catabolism, Lu Zhang
Department of Biochemistry: Dissertations, Theses, and Student Research
The role of proline metabolism in regulating cellular redox status was first proposed three decades ago. Proline catabolism was then later found to induce programmed cell death and cell apoptosis by regulating ROS signaling. Proline oxidation was also found to promote cell survival under oxidative stress. Proline catabolism-mediated reactive oxygen species (ROS) were suggested to be involved in both cases by serving as a regulatory signal. In this work, the sources of proline oxidation-induced ROS production were explored in both bacteria and animal cells. Proline oxidation-induced ROS was found to be shared by bacteria (Escherichia coli) and animals …
Investigation Of Pathways For Complex Sphingolipid Biosynthesis In Arabidopsis Thaliana (L.) Heynh, Kyle Luttgeharm
Investigation Of Pathways For Complex Sphingolipid Biosynthesis In Arabidopsis Thaliana (L.) Heynh, Kyle Luttgeharm
Department of Biochemistry: Dissertations, Theses, and Student Research
Sphingolipids are essential components of eukaryote membranes. The ceramide backbone of complex sphingolipids is composed of an 18 carbon Long Chain Base (LCB) bound to a 16-26 carbon fatty acid (FA) through an amide linkage. Ceramides are synthesized de novo from a free LCB and fatty acyl coA by ceramide synthase (sphingosine N-acyl transferase, EC 2.3.1.24) which can be inhibited by the fungal mycotoxin Fumonisin B1. Arabidopsis thaliana contains three ceramide synthases denoted LOH1, LOH2, and LOH3 that have previously been hypothesized to have unique substrate preferences that control the final sphingolipid composition, different susceptibilities to …
Characterization Of Inhibitors Of Fatty Acid Transport Protein-2 In Cell And Animal Models, Nipun Saini
Characterization Of Inhibitors Of Fatty Acid Transport Protein-2 In Cell And Animal Models, Nipun Saini
Department of Biochemistry: Dissertations, Theses, and Student Research
Obesity is correlated with insulin resistance and elevated levels of glucose, triglycerides and free fatty acids in blood. This affects overall metabolism and leads to disease. In the obese state, fat also accumulates in non-adipose tissue including liver, muscle and pancreas, where it can lead to cellular dysfunction and death. Currently, only a limited number of drugs are available to combat obesity and it is clear that new drugs, which more narrowly target the metabolic pathways involved, are required. Fatty Acid Transport Proteins (FATPs) are bifunctional proteins involved in the uptake and activation of fatty acids by esterification with coenzyme …
Functional Genomic Analyses Of Switchgrass Developmental Processes, Nathan Palmer
Functional Genomic Analyses Of Switchgrass Developmental Processes, Nathan Palmer
Department of Biochemistry: Dissertations, Theses, and Student Research
Switchgrass (Panicum virgatum L.), a C4-perennial grass species, is being developed as a bioenergy crop. Although much is known from a breeding perspective, there is limited information on the functional genomics of this crop, specifically regarding molecular mechanisms controlling aerial senescence, winter dormancy, and traits that confer winter hardiness. Using functional genomics to generate a transcriptional roadmap underpinning senescence and winter dormancy will provide researchers with a molecular understanding that can be applied to improve switchgrass germplasm.
In an initial study, a de novo assembly of the crown and rhizome transcriptome from an upland cultivar Summer was …