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Articles 1 - 9 of 9
Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Identification Of Soybean Lipoxygenase Products By Gas Chromatography-Mass Spectrometry (Ge-Ms), Rehan Ahmad Malik
Identification Of Soybean Lipoxygenase Products By Gas Chromatography-Mass Spectrometry (Ge-Ms), Rehan Ahmad Malik
Journal of Undergraduate Research at Minnesota State University, Mankato
Lipoxygenases (LOXs) are enzymes which catalyze peroxidation of polyunsaturated fatty acids containing at least one cis, cis 1, 4-pentadiene moiety to form conjugated diene-hydroperoxides. Soybean seeds contain three LOX isoenzymes while at least five different isoenzymes are in the vegetative tissue. Lipoxygenases have been related to several functions i.e. plant growth, defense mechanisms against pathogens and pests, and lipid metabolism. Given the proposed roles of LOX isoenzymes and the presence of multiple LOX isoenzymes in soybean vegetative tissue, it is likely that individual isoenzymes play specific functions. Do the isoenzymes show differences in which products they form? The basic goal …
The Effect Of Maternal Aldosterone Levels On The Expression Of 11b-Hsd Isoenzymes In Normal And Hypertensive Rat Placentae, Nicole Jorissen
The Effect Of Maternal Aldosterone Levels On The Expression Of 11b-Hsd Isoenzymes In Normal And Hypertensive Rat Placentae, Nicole Jorissen
Journal of Undergraduate Research at Minnesota State University, Mankato
In this project, we developed a Western blotting procedure to semi-quantitate levels of 11β-HSD1 and 11β-HSD2 in whole cell extracts. Then, we applied this technique to analyze the effect of reduced maternal aldosterone levels on the expression of 11β-HSD1 and 11β-HSD2 isoenzymes in the placental tissue in both normal and hypertensive rats. These enzymes control levels of glucocorticoids which compete for aldosterone’s mineralocorticoid receptor. Overstimulation of this receptor results in hypertension. If aldosterone levels decrease, levels of the enzymes controlling active glucocorticoid concentrations might change to compensate for the lowered aldosterone levels. Decreased placental 11β-HSD2 expression could affect hypertension in …
Evaluation Of Pretreatment Methods In The Production Of Ethanol From Cattail Leaves, Kristen Krahmer, Elijah Wreh
Evaluation Of Pretreatment Methods In The Production Of Ethanol From Cattail Leaves, Kristen Krahmer, Elijah Wreh
Journal of Undergraduate Research at Minnesota State University, Mankato
Previous research in this lab indicated that cattails are a potential source of biomass for the production of cellulosic ethanol since their carbohydrate composition is comparable to that of other plants being considered for biofuel production. To further test their viability, we tested various pretreatment methods on dried cattail leaves. Before polysaccharides in plants can be enzymatically hydrolyzed to fermentable sugars, the plant material must be pretreated to render the polysaccharides accessible to the enzymes. The purpose of this project has been to compare the efficiency of sulfuric acid and ammonia pretreatment methods in preparing cattail biomass for ethanol production. …
Localization And Expression Level Of Vascular Endothelial Growth Factor After Partial Hepatectomy Of Wky Rats, Adam Kronebusch, Tyler Koonst
Localization And Expression Level Of Vascular Endothelial Growth Factor After Partial Hepatectomy Of Wky Rats, Adam Kronebusch, Tyler Koonst
Undergraduate Research Symposium
Inflammation and fibrosis are indicative of liver regeneration following injury and chronic liver diseases, such as cirrhosis and hepatocellular carcinoma. The formation of new vasculature via the process of angiogenesis is vital to the pathological progression of liver regeneration and these diseases. Thus, inhibiting the process of angiogenesis could have the potential to stop or slow the progression of chronic liver diseases. Vascular endothelial growth factor (VEGF) is the most potent and specific growth factor for initiating the process of angiogenesis, making it a prime target to inhibit angiogenesis. Bevacizumab is an antibody that binds to VEGF, inhibiting it from …
Estrogenic Effects On A Protandrous Hermaphroditic Species: Amphiprion Percula, Randall Burns, Katie Dose
Estrogenic Effects On A Protandrous Hermaphroditic Species: Amphiprion Percula, Randall Burns, Katie Dose
Undergraduate Research Symposium
Estrogen concentrations are increased in aquatic environments due to the addition of pharmaceutical drugs (i.e. birth control) through runoff water. This manipulates maturation of sexes within many species that inhabit aquatic enviornments, including fresh and marine habitats. Amphiprion percula exhibit a hierarchal system behavioral pattern, which determines the social status of individuals within a group. Multiple clown anemonefish, A. percula were studied via a controlled environment and hierarchal succession was observed under estrogenic additions. From this study, data on sex change of a hermaphroditic protandrous species was observed under the influence of three concentrations of natural estrogen. Based on sex …
Rubidium Uptake In Isolated Rat Cardiomyocytes, Jooyoung Lee, Jaekook Sim, Karleen Doering
Rubidium Uptake In Isolated Rat Cardiomyocytes, Jooyoung Lee, Jaekook Sim, Karleen Doering
Undergraduate Research Symposium
The element rubidium is known to have similar biological impact as K+ ions and some studies have shown that heart tissue takes up rubidium through K+ channels and Na+/K+ ATPase pumps. The purpose of the research was to confirm whether or not rubidium was transported into individual cardiomyocytes. Rubidium may be used as a marker to study different physiological functions of K+ and its channels and pumps. The methods used are mainly two parts: isolation of single heart cells and treatment with rubidium. Isolated heart cells were obtained by anesthetizing a rat, extracting the heart, and infusing its aorta with …
Effect Of Artificial Sweeteners On The Renin-Angiotensin System In Rats, Jacob Ball
Effect Of Artificial Sweeteners On The Renin-Angiotensin System In Rats, Jacob Ball
Undergraduate Research Symposium
Normal abundant dietary sugars such as fructose and glucose can contribute to hypertension and other health issues. To avoid these health complications, many individuals use artificial sweeteners. An equivalent intake of some artificial sweeteners also can lead to hypertension. However, Stevia, a sweetener that is isolated from a Paraguayan plant, was shown in relevant literature to decrease blood pressure in both rat specimens and humans. The general purpose of this research project was to study the effect of Stevia and glucose on the expression of two key components of the renin-angiotensin-aldosterone system (RAAS): prorenin receptor (PRR) and angiotensin receptor type …
Quorum Sensing Molecules For Unicellular Organisms: Spectroscopic And Computational Study Of Conformational Behavior, Daniel Tollefson
Quorum Sensing Molecules For Unicellular Organisms: Spectroscopic And Computational Study Of Conformational Behavior, Daniel Tollefson
Undergraduate Research Symposium
Quorum sensing plays a vital role in unicellular communications. Until recently, it was thought that unicellular bacteria were non-cooperative; that they did not communicate among each other as a larger community. They do, in fact, communicate via small molecules that are created and released into the extracellular environment. Detailed knowledge regarding the interactions of these quorum sensing molecules (QSM) with the molecular and cellular-scale environments can lead to the manipulation of quorum sensing within a population. The function of these molecules requires that they can readily diffuse through the polar environment of aqueous solution and the nonpolar environment of cell …
High Fat Diet Increased Serum Glutamate Dehydrogenase More Than Chronic Acetaminophen Dosing In Female Mice, Elizabeth Ruth Behmer
High Fat Diet Increased Serum Glutamate Dehydrogenase More Than Chronic Acetaminophen Dosing In Female Mice, Elizabeth Ruth Behmer
All Graduate Theses, Dissertations, and Other Capstone Projects
This laboratory study examined the effects of acetaminophen overdose in normal fed and high-fat fed female mice. Forty female mice were placed on normal and high-fat diets at 4 weeks old. When the mice were significantly different in weight (between 6 and 9 months old), half the mice were dosed with acetaminophen. These mice were daily given an overdose of acetaminophen for 14 days. The dose used was 300 mg/kg mouse (LD50 = 338 mg/kg). The control group was given 10 µl water/g mouse. Levels of serum glutamate dehydrogenase (GDH) were measured to indicate liver damage. GDH is released from …