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Biochemistry, Biophysics, and Structural Biology Commons™
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- (β-Halo)vinylsulfone (1)
- Aptamers (1)
- Aromatic disulfides (1)
- Arsinothricin (1)
- BPTI (1)
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- Biosensors (1)
- Drug Forensics (1)
- Folding pathway (1)
- Forensic Electrochemistry (1)
- GSSG and GSH (1)
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- Nanomatericals (1)
- Opioids (1)
- Paper Electrodes (1)
- Paper-based Sensing (1)
- Polyaromatic Hydrocarbons (1)
- Protein folding (1)
- Purine Nucleosides (1)
- Sweat sensing (1)
- Β-Ketosulfone (1)
Articles 1 - 3 of 3
Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Aptamer-Based Voltammetric Biosensing For The Detection Of Codeine And Fentanyl In Sweat And Saliva, Rosa Lashantez Cromartie
Aptamer-Based Voltammetric Biosensing For The Detection Of Codeine And Fentanyl In Sweat And Saliva, Rosa Lashantez Cromartie
FIU Electronic Theses and Dissertations
Despite the many governmental and medicinal restrictions created to combat the opioid epidemic in the United States, opioid abuse and overdose rates continue to rise. The development of an aptamer-based voltammetric sensor and biosensor is described in this dissertation. The aim was to develop a low-cost, sensitive, and specific aptamer-based sensor for on-site, label-free determination of codeine and fentanyl in biological fluids. To do this, the surfaces of screen-printed carbon electrodes (SPCE) were modified with gold nanoparticles (AuNPs), followed by the addition of single-stranded DNA aptamers. These were covalently bound to the electrode surface. Operations of the sensors were collected …
Purine Nucleosides Modified At C8 Or C2 Position With (Β-Halo)Vinylsulfone And Β-Ketosulfone Reactive Groups And Their Incorporation Into Dna: Synthesis Of The Organoarsenical Antibiotic Arsinothricin And Polyaromatic Hydrocarbons, Md Abu Hasan Howlader
FIU Electronic Theses and Dissertations
Modified nucleosides gained great attention as potential anticancer and antiviral therapeutics. In this dissertation, synthesis and reactivity of (β-iodovinyl)sulfone and β-ketosulfone groups incorporated into purine nucleosides at C8 or C2 positions and DNA incorporation of their 5' triphosphates have been developed. Moreover, synthesis of novel antibiotic arsinothricin (AST) as well as polycyclic aromatic hydrocarbons (PAHs) have been discussed. The 8-(1-iodo-2-tosylvinyl)-2'-deoxyadenosine and 8-(1-Iodo-2-tosylvinyl)adenosine were synthesized employing iodovinylsulfonation of 8-ethynyl precursors with TsNa/I2/NaOAc. The 8-(β-iodovinyl)sulfonyl-2'-deoxyguanosine was prepared via radical mediated iodovinylsulfonation of 8-ethynyl-2'-deoxyguanosine with TsNHNH2/KI/(BzO)2. Conformationally different C2 substituted isomeric adenosine analogues were prepared by iodovinylsulfonation …
Investigating The In Vitro Oxidative Folding Pathways Of Bovine Pancreatic Trypsin Inhibitor (Bpti), Yingsong Wang
Investigating The In Vitro Oxidative Folding Pathways Of Bovine Pancreatic Trypsin Inhibitor (Bpti), Yingsong Wang
FIU Electronic Theses and Dissertations
The oxidative folding pathway of the disulfide containing protein bovine pancreatic trypsin inhibitor (BPTI) was one of the first to be elucidated and has served as a basis for understanding the folding pathways of other proteins. During the oxidative folding of reduced BPTI, two intermediates (N' and N*) accumulate in significant amounts and act as kinetic traps. Both N' and N* bury their two remaining free thiols in their hydrophobic cores, which inhibits further oxidation. Historically, the rate limiting step was considered to be the intramolecular rearrangements of N' and N* to another intermediate with two free thiols, NSH …