Open Access. Powered by Scholars. Published by Universities.®
Biochemistry, Biophysics, and Structural Biology Commons™
Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Methods To Assess The Direct Interaction Of C. Jejuni With Mucins, Julie Ann Naughton, Marguerite Clyne, Gina Duggan, Billy Bourke
Methods To Assess The Direct Interaction Of C. Jejuni With Mucins, Julie Ann Naughton, Marguerite Clyne, Gina Duggan, Billy Bourke
Books/Book chapters
Studies of the interaction of bacteria with mucus secreting cells can be complemented at a more mechanistic level by exploring the interaction of bacteria with purified mucins. Here we describe a far western blotting approach to show how C. jejuni proteins separated by SDS PAGE and transferred to a membrane or slot blotted directly onto a membrane can be probed biotinylated mucin. In addition we describe the use of novel mucin microarrays to assess bacterial interactions with mucins in a high throughput manner.