Biochemistry, Biophysics, and Structural Biology Commons^™

The Role Of Liver Sinusoidal Endothelial Cells In Liver Malady Homeostasis, Fatima Cabral

Department of Biochemistry: Dissertations, Theses, and Student Research

Current literature described techniques for the purification of liver cell types through text alone. The techniques described for the isolation of liver sinusoidal endothelial cells as well as hepatocytes described here are modified from a published article in the Journal of Visualized experiments. The video protocol allows for the user to successfully isolate cells as the most difficult parts of the procedure are demonstrated visually. The detection of liver maladies such as the non-alcoholic fatty liver disease, the stage if this disease and differentiation between non-alcoholic and alcoholic fatty liver disease is demonstrated in the development of a unique panel …

Er-Associated Degradation And Cadmium Dependent Rescue Of Pca1, Nathan Smith

Department of Biochemistry: Dissertations, Theses, and Student Research

Protein synthesis and proper folding is an essential process for all organisms. In eukaryotes proteins of the secretory pathway are synthesized and inserted into the lumen or membrane of the endoplasmic reticulum. Eukaryotic cells maintain a mechanism for removal of proteins unable to fold properly. This process is known as ER-associated degradation (ERAD). A poorly functioning ERAD can lead to a build-up of misfolded proteins which has been implicated in several degenerative diseases such as Alzheimer’s, Amyotrophic lateral sclerosis, and Parkinson’s. Thus, the study of how proteins are recognized, extracted from the ER, and degraded is essential for determining methods …

Studies On Nutritional Muscular Dystrophy. I. Dietary Factors, Ii. Fibrosis And Lipomatosis Of Tissues, Violet Myrtle Wilder

Department of Biochemistry: Dissertations, Theses, and Student Research

Dissertation published as three peer-reviewed papers:

Sergius Morgulis, Violet M. Wilder, and S. H. Eppstein. (September 1938). Further studies on dietary factors associated with nutritional muscular dystrophy. Journal of Nutrition 16(3): 219-227.

Howard C. Spencer, Sergius Morgulis, and Violet M. Wilder. (August 1937). A micromethod for the determination of gelatin and a study of the collagen content of muscles from normal and dystrophic rabbits. Journal of Biological Chemistry 120(1): 257-266.

Sergius Morgulis, Violet M. Wilder, Howard C. Spencer, and S. H. Eppstein. (August 1938). Studies on the lipid content of normal and dystrophic rabbits. Journal of Biological Chemistry 124(3): 755-766.