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Determination Of The Sivmac Vif-Human Apobec3b Interaction, Oumar Sanogo

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The APOBEC3 family of enzymes are DNA cytosine deaminases, some of which restrict replication of HIV-­‐1. This viral restriction is caused by deamination of cytosines to uracils in the viral cDNA, resulting in lethal mutation. HIV-­‐1 counteracts this deamination by producing the protein Vif, which targets the restricting APOBEC3 enzymes for proteosomal degradation. Previous studies have demonstrated that HIV-­‐1 Vif mediates degradation of APOBEC3D (A3D), APOBEC3F (A3F), APOBEC3G (A3G), and APOBEC3H (A3H). Other lentiviruses may also encode a Vif protein, however not all Vif proteins can degrade the same APOBEC3 proteins. For example, SIVmac (simian immunodeficiency virus that infects rhesus …