Biochemistry, Biophysics, and Structural Biology Commons^™

Nano- And Micro-Second Electrical Pulsing Of B16-F10 Mouse Melanoma Cells: Plasma Membrane And Sub-Cellular Organelle Changes, Yiling Chen

Theses and Dissertations in Biomedical Sciences

High electric field-treated cells are permeable to molecular dye through either opening of pores in the plasma membrane or other unknown processes which can disturb the membrane in an organized way. However, direct morphological evidence is lacking and responses of intracellular organelles are not clear. We used traditional chemical fixatives and biochemical techniques to capture cell membrane and organelle changes immediately after pulsing with high voltage electric field application. Different pulse durations, nanosecond (ns) and microsecond (µs), and field magnitudes, 60 kV/cm and 1.2 kV/cm, were applied to mouse melanoma B16-F10 cells. Two different ns durations (60 and 300 ns) …