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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
The Metabolic Change Of Lipid Species In Stam Mice With Hepatocellular Carcinoma, Devin J. Peart
The Metabolic Change Of Lipid Species In Stam Mice With Hepatocellular Carcinoma, Devin J. Peart
UNLV Theses, Dissertations, Professional Papers, and Capstones
Primary liver cancer hepatocellular carcinoma (HCC) is the second leading cause of cancer death worldwide. In the US, primary liver cancer is the most rapidly increasing type of cancer due to increases in hepatitis B and C and the continued trend of greater and greater percentages of the population suffering from obesity, diabetes mellitus and metabolic syndrome. The prognosis for this disease is poor, with a 5-year survival rate of 26% as compared to only 2% when the cancer is metastatic. Unfortunately, current strategies for early detection of HCC, including routine CT imaging or alpha-fetoprotein biomarker, are suboptimal and tend …
Development And Application Of Mass Spectrometry-Based Protein Footprinting In Structural Proteomics, Ming Cheng
Development And Application Of Mass Spectrometry-Based Protein Footprinting In Structural Proteomics, Ming Cheng
Arts & Sciences Electronic Theses and Dissertations
Integral mass spectrometry (MS) has emerged as an important tool for protein structural characterization. It readouts are a broad range of structural information, including stoichiometry, interactions, conformations and conformation change, and dynamics. Protein footprinting is a pivotal component in the intergral MS toolkit.My dissertation centers around the development and application of protein footprinting to characterize protein structure. It is divided into seven chapters.Chapter 1 serves as the introduction for integral mass spectrometry in structural proteomic.In Chapter 2, we extended the fast-photochemical oxidation of proteins (FPOP) platform by adding the trifluoromethyl radical (•CF3) as a new reagent. We discovered that •CF3 …