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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Developing Sodium Mri For Longitudinal Assessment Of Chemotherapy Of Glioma In A Rat Model Of Cancer, Xin Yue Wang
Developing Sodium Mri For Longitudinal Assessment Of Chemotherapy Of Glioma In A Rat Model Of Cancer, Xin Yue Wang
Electronic Thesis and Dissertation Repository
Glioblastoma (GBM) is an aggressive cancer which presents significant challenges for disease management and treatment. Tissue sodium concentration (TSC) is a sensitive indicator of disease and changes in TSC can be used as a potential biomarker for treatment response. A longitudinal orthotopic C6 GBM model was used to study changes in TSC due to cancer. Animals were imaged with contrast enhanced T1-weighted, T2-weighted and sodium MRI and were either un-treated or received temozolomide (TMZ) treatment. Accurate assessment of TSC was possible for tumour volumes greater than 150mm3 using 3mm isotropic imaging voxels. TSC increased with …
Monocyte Mri Relaxation Rates Are Regulated By Extracellular Iron And Hepcidin, Praveen S.B Dassanayake
Monocyte Mri Relaxation Rates Are Regulated By Extracellular Iron And Hepcidin, Praveen S.B Dassanayake
Electronic Thesis and Dissertation Repository
Monocytes are an important immune cell type in chronic inflammatory conditions like atherosclerosis and heart failure. The increase in number of monocytes released to the peripheral blood circulation, the differentiation of monocytes to macrophages, and the presence of different macrophage subpopulations during pro- and anti-inflammatory stages of tissue injury may provide markers for monitoring inflammation. In particular, changes in monocyte iron metabolism during an inflammatory response may increase the possibility of tracking these immune cells non-invasively using magnetic resonance imaging (MRI). When secretion of the polypeptide hormone hepcidin is stimulated during inflammation, it binds the iron export protein ferroportin (FPN) …
Towards The Development Of A Maga Reporter Gene Expression Construct For Magnetic Resonance Imaging, Karina Quiaoit
Towards The Development Of A Maga Reporter Gene Expression Construct For Magnetic Resonance Imaging, Karina Quiaoit
Electronic Thesis and Dissertation Repository
Development of methods to monitor cellular activity with magnetic resonance imaging (MRI) provides a powerful tool for studying disease progression and monitoring therapy. This thesis investigates components necessary for development of reporter gene expression for MRI, to track Nkx2.5 transcription factor activity during tumour growth and, in the future, to calibrate MR contrast against a well-known optical reporter gene, like firefly luciferase. In human MCF-7 breast cancer cells, Nkx2.5 is induced by all-trans retinoic acid (tRA) and activates a minimal promoter of the rat sodium iodide symporter (rNISmin) gene. Here, we used firefly luciferase reporter gene expression to examine a …