Biochemistry, Biophysics, and Structural Biology Commons^™

Ets-Core Binding Factor: A Common Composite Motif In Antigen Receptor Gene Enhancers, Batu Erman, Marta Cortes, Barbara S. Nikolajczyk, Nancy A. Speck, Ranjan Sen

Dartmouth Scholarship

A tripartite domain of the murine immunoglobulin μ heavy-chain enhancer contains the μA and μB elements that bind ETS proteins and the μE3 element that binds leucine zipper-containing basic helix-loop-helix (bHLH-zip) factors. Analysis of the corresponding region of the human μ enhancer revealed high conservation of the μA and μB motifs but a striking absence of the μE3 element. Instead of bHLH-zip proteins, we found that the human enhancer bound core binding factor (CBF) between the μA and μB elements; CBF binding was shown to be a common feature of both murine and human enhancers. Furthermore, mutant enhancers that bound …

Collaborative Roles For C-Jun N-Terminal Kinase, C-Jun, Serum Response Factor, And Sp1 In Calcium-Regulated Myocardial Gene Expression, Patrick M. Mcdonough, Deanna S. Hanford, Amy B. Sprenkle, Noel R. Mellon, Christopher C. Glembotski

Amy Sprenkle

Ms2 Coat Protein Mutants Which Bind Qβ Rna, Marc Spingola, David Peabody

Biology Department Faculty Works

The coat proteins of the RNA phages MS2 and Qβ are structurally homologous, yet they specifically bind different RNA structures. In an effort to identify the basis of RNA binding specificity we sought to isolate mutants that convert MS2 coat protein to the RNA binding specificity of Qβ. A library of mutations was created which selectively substitutes amino acids within the RNA binding site. Genetic selection for the ability to repress translation from the Qβ translational operator led to the isolation of several MS2 mutants that acquired binding activity for Qβ RNA. Some of these also had reduced abilities to …

Ms2 Coat Protein Mutants Which Bind Qβ Rna, Marc Spingola, David S. Peabody

Marc Spingola

Las Vegas Wash Water Quality Monitoring Program: 1996 Report Of Findings, Richard A. Roline, James J. Sartoris, U.S. Bureau Of Reclamation, U.S. Geological Survey

Publications (WR)

Las Vegas Wash, a natural wash east of the city of Las Vegas, Nevada, carries stormwater, groundwater drainage, and sewage effluent from three sewage treatment plants to Lake Mead. The Wash provides nearly the only surface water outlet for the entire 2,193 mi² of Las Vegas Valley. A drainage area of 1,586 mi² contributes directly to the Wash through surface flow which is channeled to Las Vegas Bay of Lake Mead, while drainage of the remaining 607 mi² is presumably subsurface and may drain toward Las Vegas Wash.

In the 1930's and 1940's, sewage treatment plants were …

Endosome To Golgi Retrieval Of The Vacuolar Protein Sorting Receptor, Vps10p, Requires The Function Of The Vps29, Vps30, And Vps35 Gene Products, Matthew N. J. Seaman, Eric G. Marcusson, Joan Lin-Cereghino, Scott D. Emr

College of the Pacific Faculty Articles

Mutations in the S. cerevisiae VPS29 and VPS30 genes lead to a selective protein sorting defect in which the vacuolar protein carboxypeptidase Y (CPY) is missorted and secreted from the cell, while other soluble vacuolar hydrolases like proteinase A (PrA) are delivered to the vacuole. This phenotype is similar to that seen in cells with mutations in the previously characterized VPS10 and VPS35 genes. Vps10p is a late Golgi transmembrane protein that acts as the sorting receptor for soluble vacuolar hydrolases like CPY and PrA, while Vps35p is a peripheral membrane protein which cofractionates with membranes enriched in Vps10p. The …

Effects Of Creatine Supplementation On Muscular Strength And Power Development In Well-Trained Football Players, Josef D. Mcquain

Theses, Dissertations and Capstones

The purpose of this study was to determine the effects of creatine supplementation on strength and power performance in well-trained football players. Twenty-one subjects were randomly assigned to three treatment groups: 1) creatine monohydrate, 2) creatine monohydrate+dextrose, or 3) placebo utilizing a double-blind design. Prior to assignment, subjects were matched according to lean body mass. After a five day loading dose, a maintenance dose was administered for 12 days. Test protocols included three anaerobic work bouts: l) two 30 s Wingate bike tests interspersed with five minutes recovery were used to ascertain relative and absolute power, 2) two 100 yard …

In Vivo Incorporation Of Tritium To Measure Lipogenesis In Red Skeletal Muscle: Significance Of A Nonlipogenic Diet In La/N-Fa(K)("Corpulent") And Zucker Fa Rat Strains, Karin Traci Mann

Theses, Dissertations and Capstones

Skeletal muscle lipogenesis in rodents may equal that of liver but its importance in the obese animals is not known. We evaluated the rate of fatty acid and triglyceride synthesis and storage content in red skeletal muscles, liver, inguinal fat, and retroperitoneal fat of the LA/N-fa^k (“corpulent”) and Zucker fa obese rat strains. Obese-lean pairs of both rat strains, weaned at 32 days, fed a nonlipogenic diet (54% starch for 21 days) were either dosed with 2 µCi 3H-H² O per gram of body weight and tested after 60 minutes for whole body 3H-incorporation into fatty acids and …

Regulation Of The Expression Of Hmn-Sod At The Level Of The Mitochondrial Processing Of Precursor Hmn-Sod, Gary Leslie Wright

Theses, Dissertations and Capstones

The mitochondrion imports and processes the vast majority of the proteins which comprise its structural elements and metabolic pathways. Studies in the last decade have been successful at defining the mechanism and protein machinery responsible for recognizing, importing, and processing cytosolic precursor proteins into the mature mitochondrial proteins. Little is known, however, about physiological factors which might influence or compromise mitochondrial protein processing. The objective of this work was to develop a novel approach to study mitochondrial import and assembly of precursor proteins as a process within the context of the cellular environment. To these ends we employed the baculovirus …

Relationship Of Resting And Submaximal Cardiovascular Measures To Vo[2]Max In Untrained Children, Thomas A. Cappaert

Theses, Dissertations and Capstones

Participation by children in organized sport continues to increase. A 1994 survey of 10,000 households by the National Sporting Goods Association (1994) found that approximately 40,000 children between the ages of 7-17 years participate in an organized sport or recreational activity annually. In the realm of organized sports, especially endurance activities, there continues to be interest in how cardiovascular fitness is attained and how it can be maximized. Measurement of cardiovascular fitness is an important piece to that puzzle.

The most common and most descriptive measure for children and adults, is the maximal oxygen uptake or max (Astrand & Rodahl, …

Description Of Tadpoles Of Three Species Of Scinax (Anura: Hylidae), Rafael O. De Sá, Rocjard Wassersig, Arturo I. Kehr

Biology Faculty Publications

Larval morphological characteristics have been used to cluster species of Scinax in seven species groups and to support the monophyly of some of these groups. However, our present knowledge of Scinax tadpoles is incomplete; currently, tadpoles of only 21 of the approximately 80 recognized species have been described. Herein, we describe and illustrate the external morphology and oral disc characteristics of larval Scinax berthae, S. nebulosa, and S. boesemani. The presence of a "labial arm" supporting the P3 tooth row in S. boesemani, and its absence from S. nebulosa, is discussed in the light of our limited knowledge of tadpoles …

Small Miracles: Propagation Efforts Using Plants In The Family Hamamelidaceae, Martha Sasser Joiner

Legacy ETDs

Attempts were made to propagate five species in the family Hamamelidaceae by softwood cuttings or seeds. Local populations of witch-hazel Hamamelis virginiana L. and sweetgum Liquidambar styraciflua L. supplied cuttings and seeds of these native American species. Cultivated plants of dwarf witch-alder Fothergilla gardenii Murray were used initially for cuttings. Later, three native populations of this species were located and cuttings were obtained from them. Also, for seed propagation studies, seeds of F. gardenii were obtained from the North Carolina Botanical Garden and seeds were obtained from cultivated plants of two Asian species, winter hazel Corylopsis sinensis Hemsley and Chinese …

Acute And Chronic Exposure To Lead: Cellular And Behavioral Effects On The Pond Snail Helisoma Trivolvis, Virginia T. Bennett

Legacy ETDs

The focus of this study is on the effects of lead on Helisoma trivolvis, a pulmonate pond snail. Many species of molluscs are being used as bioindicators of environmental toxins. To provide a background for the reader, a brief history and description of the Phylum Mollusca (at the class level) is presented in Chapter 1. A second chapter (Ch. 2) is concerned with lead and its effects. A synopsis of past and present studies that show the interaction between lead and molluscs is given in Chapter 3, then two experimental chapters follow. The first experimental chapter (Ch. 4) is …

Nest Box Use, Reproductive Success, And Nestling Growth Of American Kestrels (Falco Sparverius) Nesting Along The Coastal Plain Of Georgia, Walter Roy Shuford

Legacy ETDs

In an attempt to increase breeding populations of American Kestrels (Falco sparverius) in Georgia, 402 nest boxes were erected in the Coastal Plain of Georgia beginning in 1994 through the spring of 1996. Nest box occupancy averaged 8% in 1996 and 9.7% in 1997, two fold the average in 1995. Occupancy at Fort Gordon military base averaged 27.5% (28 of 102) in 1996 and 29 4% (30 of 102) in 1997 Nest box use in all other areas averaged 13% (4 of 300) in 1996 and increased to 3% (9 of 300) in 1997. Clutch sizes for nests …

The Pax-5 Gene Is Alternatively Spliced During B-Cell Development, Patty Zwollo, Hector Arrieta, Kaleo Ede, Karen Molinder, Stephen Desiderio, Roberta Pollock

Roberta Pollock

The transcription factor Pax-5 is expressed during the early stages of B-cell differentiation and influences the expression of several B-cell-specific genes. In addition to the existing isoform (Pax-5, which we have named Pax-5a), we have isolated three new isoforms, Pax-5b, Pax-5d, and Pax-5e, from murine spleen and B-lymphoid cell lines using library screenings and polymerase chain reaction amplification. Isoforms Pax-5b and Pax-5e have spliced out their second exon, resulting in proteins with only a partial DNA-binding domain. Isoforms Pax-5d and Pax-5e have deleted the 3′-region, which encodes the transactivating domain, and replaced it with a novel sequence. The existence of …