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Poultry or Avian Science Commons

Open Access. Powered by Scholars. Published by Universities.®

Tennessee State University

2015

Articles 1 - 1 of 1

Full-Text Articles in Poultry or Avian Science

Molecular Tools To Support Metabolic And Immune Function Research In The Guinea Fowl (Numida Meleagris), Carl E. Darris, James E. Tyus Ii, Gary Kelley, Alexander J. Ropelewski, Hugh B. Nicholas Jr., Xiaofei Wang, Samuel N. Nahashon May 2015

Molecular Tools To Support Metabolic And Immune Function Research In The Guinea Fowl (Numida Meleagris), Carl E. Darris, James E. Tyus Ii, Gary Kelley, Alexander J. Ropelewski, Hugh B. Nicholas Jr., Xiaofei Wang, Samuel N. Nahashon

Agricultural and Environmental Sciences Faculty Research

Background

Guinea fowl (Numidia meleagris) production as an alternative source of meat and poultry has shown potential for economic viability. However, there has been little progress in characterizing the transcriptome of the guinea fowl. In this study RNA-sequencing and de novo transcriptome assembly of several Guinea fowl tissues (pancreas, hypothalamus, liver, bone marrow and bursa) which play key roles in regulating feed intake, satiety, and immune function was performed using Illumina’s Hi-Seq 2000.

Results

74 million sequences were generated and assembled into 96,492 contigs using the Trinity software suite. Over 39,000 of these transcripts were found to have …