Meat Science Commons^™

Rapid Communication: Porcine Vitamin D-25-Hydroxylase Maps To Chromosome 5, Daniel C. Ciobanu, R. K. Raven, M. F. Rothschild, N. H. Bell

Department of Animal Science: Faculty Publications

Locus Name. Vitamin D-25-hydroxylase.

Species. Sus scrofa.

Locus Symbol. CYP25.

Source of Primers. PCR primers were designed from the published sequence for porcine vitamin D-25-hydroxylase (CYP25) cDNA (GenBank accession no. Y16417). The position of the forward and reverse primers corresponds to 506 to 530 bp and 830 to 854 bp, respectively, of the cDNA. The primer pair amplified a 348-bp fragment of the cDNA and an 862-bp fragment from the pig genomic DNA. The 862-bp fragment (Gen- Bank accession no. AF284378) was subcloned into a pNoTA vector and identity was confirmed by sequencing. Genomic sequences from 1 to 74, …

Comparison Of Plasma Fsh Concentration In Boars And Gilts From Lines Selected For Ovulation Rate And Embryonal Survival, And Litter Size And Estimation Of (Co)Variance Components For Fsh And Ovulation Rate, J. D. Cassady, Rodger K. Dennison, J. J. Ford

Department of Animal Science: Faculty Publications

The objective of this research was to determine whether plasma concentration of FSH was genetically correlated with ovulation rate and thus was a useful trait for indirect selection. Blood samples were collected from 619 animals from five lines of pigs. Line I was selected for increased index of ovulation rate and embryonal survival, and Line C was its randomly selected control. Pigs sampled from Lines I and C were from generations 12 and 13. Pigs from three additional lines that were derived from eighth-generation pigs of Lines I and C also were used. These lines were Line C2, a randomly …