Animal Sciences Commons^™

Genetic And Genomic Analysis Of Hyperlipidemia, Obesity And Diabetes Using (C57bl/6j × Tallyho/Jngj) F2 Mice, Taryn P. Stewart, Hyoung Y. Kim, Arnold M. Saxton, Jung H. Kim

Nutrition Publications and Other Works

Background

Type 2 diabetes (T2D) is the most common form of diabetes in humans and is closely associated with dyslipidemia and obesity that magnifies the mortality and morbidity related to T2D. The genetic contribution to human T2D and related metabolic disorders is evident, and mostly follows polygenic inheritance. The TALLYHO/JngJ (TH) mice are a polygenic model for T2D characterized by obesity, hyperinsulinemia, impaired glucose uptake and tolerance, hyperlipidemia, and hyperglycemia.

Results

In order to determine the genetic factors that contribute to these T2D related characteristics in TH mice, we interbred TH mice with C57BL/6J (B6) mice. The parental, F1, and …

Diagnosis And Management Of Horses With Equine Metabolic Syndrome (Ems), Kelly Ann Chameroy

Doctoral Dissertations

In horses, a painful and often debilitating disease known as laminitis can result in impaired function and, in severe cases, euthanasia. Equine Metabolic Syndrome (EMS) is a syndrome in horses that results in development of laminitis and is characterized by the presence of general and/or regional adiposity (“cresty neck”), aberrations in blood lipid concentrations, insulin resistance (IR) and/ or hyperinsulinemia. Therapies have focused on improving the state of obesity and insulin resistance with the goal of diminishing the likelihood of laminitis development. A definitive cause for laminitis has not been established, but hyperinsulinemia and IR are likely candidates as experimental …

Impacts Of Predation Risk And Development On Susceptibility Of North American Anurans To Ranaviruses, Nathan Alden Haislip

Masters Theses

For over three decades, amphibian populations have been declining across the globe. Emerging infectious diseases are responsible for some of these declines. Ranaviruses have caused die-offs in wild amphibian populations on 4 continents, in 5 Canadian provinces, and in over 25 U.S. states. In order to understand host-pathogen dynamics, it is critical to establish baseline information on species susceptibility and the effects of natural stressors. The goal of my thesis research was to quantify the effects of anuran development and exposure to invertebrate predators on species-specific susceptibility to ranavirus. My experiments were designed in factorial arrangements, and consisted of exposure …

“Development Of Genetic And Genomic Predictors Of Fertility In Argentinean Holstein Cattle.”, Fernando Alfonso Di Croce

Doctoral Dissertations

The overall aim of the studies described herein was to evaluate genetic variation in cattle fertility traits for development of genetic and genomic predictors in breeding strategies. Results from these experiments suggest that improvements in fertility through genetic selection are a possible approach to increase reproductive efficiency. Experiment 1 evaluated the development of genetic parameters associated with multiple ovulation and embryo transfer schemes in an attempt to assist producers in identifying animals with greater genetic merit for these protocols. This study confirmed that genetic selection of donors or sires appears to be a potential approach to improve efficiency of MOET …

The Effects Of Weaning Strategy On The Physiology And Performance Of Beef Calves, Cristina Campistol

Masters Theses

Two experiments examined growth performance and physiological measures of stress in pre- and postweaned Angus steers (313 ± 24.5 kg; n = 48/Exp.), where steers were fitted with (YD) or without (ND) an anti-suckling device (Exp. 1), or provided (YS) or not provided (NS) a grain supplement (Exp. 2) for 7 d and weaned by fenceline (FS) or total separation (TS). Steers in Exp. 1 were weighed and bled on d 0, 3, 7 10, 14, 21, and 42, and in Exp. 2, on d 0, 7, 10, 14, and 21 and provided a grain supplement on d 7-21. In …

Identifying Mechanisms Associated With Innate Immunity In Cows Genetically Susceptible To Mastitis, Alexandra Alida Elliott

Doctoral Dissertations

Mastitis, or mammary gland inflammation, causes the greatest loss in profit for dairy producers. Mastitis susceptibility differs among cows due to environmental, physiological, and genetic factors. Prior research identified a genetic marker in a chemokine receptor, CXCR1, associated with mastitis susceptibility and decreased neutrophil migration. Current research seeks to identify reasons behind mastitis susceptibility by validating this model through in vivo challenge with Streptococcus uberis and studying specific mechanisms causing impaired neutrophil migration. Holstein cows with GG (n=19), GC (n=28), and CC (n=20) genotypes at CXCR1+777 were challenged intramammarily with S. uberis strain UT888. After challenge 68% of quarters from …

Discovery Fall 2010, Misty Bailey

Discovery Research Newsletter

Discovery is a quarterly research newsletter published by the College of Veterinary Medicine Office of Research and Graduate Studies. It is intended to highlight research and sponsored programs in the college and provide information about research opportunities.

Selected Hormonal And Neurotransmitter Mechanisms Regulating, James L. Sartin, Joseph A. Daniel, Brian K. Whitlock, Robyn R. Wilborn

Faculty Publications and Other Works -- Large Animal Clinical Sciences

Appetite control is a major issue in normal growth and in suboptimal growth performance settings. A number of hormones, in particular leptin, activate or inhibit orexigenic or anorexigenic neurotransmitters within the arcuate nucleus of the hypothalamus, where feed intake regulation is integrated. Examples of appetite regulatory neurotransmitters are the stimulatory neurotransmitters neuropeptide Y (NPY), agouti-related protein (AgRP), orexin and melanin-concentrating hormone and the inhibitory neurotransmitter, melanocyte-stimulating hormone (MSH). Examination of messenger RNA (using in situ hybridization and realtime PCR) and proteins (using immunohistochemistry) for these neurotransmitters in ruminants has indicated that physiological regulation occurs in response to fasting for several …

Effect Of Double Interspiking On Fertility, Behavior, And Blood Parameters In Broiler Breeder Males Reared Under Heat Stress Conditions, Karianne Mei-Ying Chung

Masters Theses

Broiler breeders experience a natural decline in fertility levels as a flock ages. A male management practice such as double interspiking could be applied to counteract this decline. Our objective was to investigate the effects of double interspiking on flock fertility, testosterone concentrations, stress responses, and behavioral responses of broiler breeders in heat stressed environments. Two hundred and eighty-eight broiler breeder pullets and thirty-six broiler breeder roosters (Ross 708) were assigned to three groups at 21 weeks of age (WOA). All three groups were housed in slatted floor pens in which room temperature cycled from 23.8 to 30 C to …

Spatial And Temporal Relationships Between Deer Harvest And Deer-Vehicle Collisions At Oak Ridge Reservation, Tennessee, Amanda Marie Pierce

Masters Theses

The Department of Energy Oak Ridge Reservation (ORR) and the nearby adjoining City of Oak Ridge, Tennessee had experienced a rise in deer-vehicle collisions (DVCs) to the point where safety for employees and residents became a concern. I investigated the effect of hunting, land cover, road mileage, season, lunar phase, sex, and change in traffic patterns that coincide with work shifts on DVCs from 1975 - 2008. The study area was divided into grids of 1.5 km² each for administration and data recording by managing agencies. Statistical analyses were performed on the ORR (121 grids) and GIS analyses were performed …

Regional Differences In Adipose Tissue Of The Sinclair Minipig, Suzanne Lauren Booker

Masters Theses

Adipose tissue is an endocrine organ, and its homeostatic mechanisms in normal weight, overweight and obese subjects must be elucidated. We sought to determine the basal adipose tissue biology of visceral (VIF) and subcutaneous (SQF) fat depots in 8 month old Sinclair minipigs, an animal that has been shown to be physiologically similar to humans.

Metabolic analysis showed a decrease in LDL, white blood cells (WBC), and lymphocyte percentages as the minipigs aged from 6 to 8 months (p <0.0001 and = 0.0046 and 0.0165 respectively). There were no significant changes in triglycerides, HDL, VLDL, and neutrophil percentages. There was a trend in insulin increase (P=0.0722).

Microarray analysis was performed to determine transcriptome differences between VIF and SQF. When VIF was compared to SQF, expression of a …

The Challenges Of Making A Blastocyst-Stage Embryo: Impact Of Heat Stress & Technical Factors Associated With Ivp Procedures, Estanislao Peixoto

Masters Theses

It was hypothesized that technical factors associated with in vitro production (IVP) of embryos may influence rate of blastocyst development of oocytes matured at 38.5 or 41.0 C. To test this hypothesis, a retrospective meta-analysis was performed. Simple linear regression was performed to analyze continuous variables and ANOVA for categorical variables. Interactions among factors and maturation temperature on blastocyst development were analyzed using dummy regression for continuous variables, and using a factorial treatment design and ANOVA for categorical variables. Month of collection was the only variable that impacted responsiveness of ova to heat stress. Independent of maturation temperature, variables that …

An Evaluation Of The Newsletter “Beef Cattle Time” – Determining The Impacts Of “Beef Cattle Time” As Perceived By Tennessee Beef Producers, Christina L. Perez

Masters Theses

This study examined the impacts of a beef cattle newsletter, “Beef Cattle Time,” on Tennessee beef producers. The purpose of this study was to assess the impacts of “Beef Cattle Time” as perceived by Tennessee beef producers on the utilization, satisfaction, benefit, and future of this newsletter. A self-developed, seventy-six question, survey was mailed to 639 randomly selected Tennessee beef cattle producers. Two hundred seventy six (43%) participants responded. One hundred thirty-four (48.6%) reported reading “Beef Cattle Time” and 142 (51.4%) had never read “Beef Cattle Time.” The utilization of “Beef Cattle Time” as a source of information was found …

Four Aspects Of Dogwood Pollination: Insect Visitation, A Novel Approach To Identify Pollen, Floral Volatile Emission, And Tracking Parentage, Paul Raymond Rhoades

Masters Theses

Part 1: Visitation of potential pollinators to Cornus florida and C. kousa flowers was assessed in East Tennessee in 2008 and 2009. Data regarding insect visitation rates to multiple trees per location were gathered throughout the flowering period. Diurnal and seasonal variations in visitation were recorded. Pollen coverage was assessed on portions of captured insect exoskeletons that were most likely to contact the stigma. Eleven families in four insect orders were collected from C. florida and 26 families in five orders from C. kousa. The most important pollinators in eastern Tennessee were bees in the Andrenidae and Halictidae. The most …

Changes In Plasma Concentrations Of Leptin In Ewes During Pregnancy, Joseph A. Daniel, A B. Milam, M E. Gafnea, Brian K. Whitlock, D H. Keisler

Animal Science Publications and Other Works

Previous research has demonstrated circulating concentrations of leptin increase in ewes during mid pregnancy then decline in late pregnancy and early lactation. The objective of this study was to more narrowly define the timing of changes in circulating concentrations of leptin with pregnancy in ewes. Katahdin ewes (n= 19) located at latitude 34.275 and longitude -85.183 (Mount Berry, GA) were utilized. Blood samples were collected weekly via jugular veinpuncture beginning immediately before ram exposure on September 23 and continuing until 4 weeks post-lambing. Ewes were exposed to a ram fitted with a marking harness for a 63 day breeding season. …

Effect Of Leukemia Inhibitory Factor On Feed Intake And Body Temperature In Sheep, James L. Sartin, D L. Marks, Brian K. Whitlock, Joseph A. Daniel, Barbara P. Steele

Animal Science Publications and Other Works

Leukemia inhibitory factor (LIF) has been suggested to function as a potent inhibitor of feed intake in rodents. These studies were designed to determine whether LIF was found in the ovine hypothalamus and whether LIF inhibited feed intake in sheep. Sheep hypothalami were used to clone LIF to indicate presence of the gene in the hypothalamus. The sequence was similar to published data. Another group of sheep were provided intraventricular (ICV) cannulas and injected with doses of LIF at 250, 500, 1000 and 2500 ng per sheep, ICV. Feed intake was inhibited by the 1000 and 2500 ng dose (trt, …

Changes In Plasma Concentrations Of Growth Hormone And Luteinizing Hormone In Ewes Following Central And Peripheral Treatment With Kisspeptin, Brian K. Whitlock, Joseph A. Daniel, Barbara P. Steele, James L. Sartin

Animal Science Publications and Other Works

Kisspeptin (KP), a neuroendocrine regulator of gonadotropin releasing hormone, has been hypothesized as an integrator of nutrition and hormones critical to metabolism and regulation of reproduction. Recent evidence suggests growth hormone (GH) secretion may be influenced by KP. The objective of this study was to determine if the GH stimulatory effect of KP is due to actions on the hypothalamus or anterior pituitary gland in ewes. Adult ovariectomized ewes (n=8) were fitted with intracerebroventricular (ICV) cannula to facilitate central administration of experimental treatments. Ewes received one of eight treatments [four intravenously (IV) and four ICV]. Peripheral treatments [0 (Veh), 100, …

F758-Forage And Grain Testing Information Sheet, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

No abstract provided.

W156-Clinical Mastitis Evaluation, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

No abstract provided.

W155-Clinical Mastitis And Treatment Record By Time Period, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

No abstract provided.

W154-Clinical Mastitis And Treatment Record By Cow, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

No abstract provided.

W153-Forms Of Mastitis, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

No abstract provided.

W152-Estimating Losses Using Somatic Cell Counts, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

No abstract provided.

W151-Bulk Tank Milk Quality Parameters, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

Bacteria in milk, whether originating from the cow or from the environment, significantly impact the quality of dairy products and therefore consumer acceptance. Bacteria produce heatstable enzymes that can damage milk fat and milk protein. These enzymes are not affected by pasteurization and continue to cause damage in the final dairy product. High bacteria levels in raw milk result in off-flavors, bitter flavors, rancidity and reduced shelf life. The Pasteurized Milk Ordinance specifies safety standards of Grade A milk to protect public health, not to maximize product quality and shelf life. The PMO only sets limits on somatic cell count …

W150-Using Milk Somatic Cell Count Information, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

The level of mastitis infection in a dairy herd can have a significant impact on herd profitability. Losses due to mastitis include decreased milk production, increased treatment costs, discarded milk, premature culling, death, decreased genetic potential, decreased reproductive performance, load rejection due to violation of somatic cell counts (SCC) or antibiotic residues and loss of milk quality premiums (3, 9, 12). Most producers easily relate clinical mastitis with these losses. However, subclinical (or hidden) mastitis may actually cause more losses in a herd than clinical mastitis. Since the animal does not exhibit typical symptoms of the disease, the problem is …

W149-Bulk Tank Milk Quality, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

Who is responsible for the safety and quality of dairy products? Dairy producers? Processors? Retailers? Consumers? In fact, all of these parties – from farm to fork – share in this responsibility. What starts as a high-quality product on the farm can be ruined somewhere along this chain. However, a poor-quality, inferior raw product leaving the farm cannot be transformed into a safe and high-quality product for the consumer. Despite technical advances in milk processing, the safety and quality of dairy products is still determined on the farm. Pasteurization does provide a certain level of safety, but is not the …

W148-Milk Quality And The Tennessee Quality Milk Initiative, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

According to records, in 1995, there were more than 1300 dairy farms in Tennessee. In 2007, the number declined to fewer than 600 dairies (9). There are several reasons for this mass exodus from the dairy industry in Tennessee: low milk prices, shortages of qualified labor, high feed costs, high fertilizer costs, high fuel costs, etc. In addition, many dairy producers in Tennessee and in the Southern Region are at risk of losing their milk market due to below average milk quality. Milk quality continues to be a topic of intense debate in the dairy industry. Dairy producers in the …

W103- Closure & Rehabilitation Of Waste Storage Facilities, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

No abstract provided.

W098-Annual Report Template, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

No abstract provided.

W097-Permit Termination Template, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

No abstract provided.