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Animal Sciences Commons™

Open Access. Powered by Scholars. Published by Universities.®

2010

Genetics and Genomics

Selected Works

Articles 1 - 4 of 4

Full-Text Articles in Animal Sciences

Molecular Cloning And Characterization Of The Porcine Fto Promoter And Coding Regions.Pdf, Jinming Huang, Yunzhou Yang, Gang Liu, Jibin Zhang, Xinxing Dong, Ying Bai, Meiying Fang Nov 2010

Molecular Cloning And Characterization Of The Porcine Fto Promoter And Coding Regions.Pdf, Jinming Huang, Yunzhou Yang, Gang Liu, Jibin Zhang, Xinxing Dong, Ying Bai, Meiying Fang

Jibin Zhang

The fat mass and obesity associated gene (FTO) has been widely reported to be associated with fat mass or fat deposition in different species. In the present study, we cloned both promoter and codingregions of the gene in pigs with over 5 Kb of sequence for the former region and 1,596 bp for the latter region. Comparative analysis of the promoter region among 20 species including pig revealed four conserved regions that harbor transcriptional factors involved in adipose differentiation. Using a pooled DNA sequencing approach, we discovered 39 single nucleotide polymorphisms (SNPs) in the pig FTO gene and four of them were …

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Molecular Phylogenetics Of The Australian Elapid Snakes: (Serpentes: Elapidae, Hydrophiinae), Catherine J. Nock Oct 2010

Molecular Phylogenetics Of The Australian Elapid Snakes: (Serpentes: Elapidae, Hydrophiinae), Catherine J. Nock

Dr Catherine J Nock

Evolutionary relationships within the Elapidae have been examined using a wide range of morphological and molecular datasets. Historically, there has been little consensus regarding relationships within the largest of the elapid subfamilies, the Hydrophiinae (sensu Slowinski et al. 1997). This presumed monophyletic group includes the sea kraits (Laticauda), the viviparous sea snakes and the terrestrial Australasian elapida. To examine relationships within this subfamily, mitochondrial DNA (partial 12S rRNA) sequence data were obtained for 19 elapid genera including an African, an Asian, 14 terrestrial Australian, a sea krait (Laticauda) and 2 viviparous sea snake genera.

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3hsds1和17hsds7基因5'上游区转录活性研究.Pdf, Xinxing Dong, Yi Xin, Ying Bai, Yunzhou Yang, Jibin Zhang, Meiying Fang, Gang Chen Dec 2009

3hsds1和17hsds7基因5'上游区转录活性研究.Pdf, Xinxing Dong, Yi Xin, Ying Bai, Yunzhou Yang, Jibin Zhang, Meiying Fang, Gang Chen

Jibin Zhang

3β-hydroxysteroid dehydrogenases (HSDs) and 17βHSDs are pivotal enzymes involved in synthesis, activation and deactivation of steroid hormones. Therefore, they play an important role to maintain homeostasis of hormone and regulate the synthesis and metabolism of hormones, and they are also related to metabolism of androstenone which can cause boar taint in male pigs. Through ligation of different PCR fragments of these genes from Large White pigs to pGL3-basic plasmid and subsequent transient transfection of constructed vectors into HepG2 cell culture, we found that the fragment from -1038bp to 45bp in porcine 3βHSD gene and the fragment from -966bp to 39bp …

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Polyphyly Of The Pikeminnows (Teleostei: Cyprinidae) Inferred Using Mitochondrial Dna Sequences, T. Heath Ogden Dec 2009

Polyphyly Of The Pikeminnows (Teleostei: Cyprinidae) Inferred Using Mitochondrial Dna Sequences, T. Heath Ogden

T. Heath Ogden

The phylogenetic relationships of the Colorado pikeminnow Ptychocheilus lucius, northern pikeminnow P. oregonensis, Sacramento pikeminnow P. grandis, Umpqua pikeminnow P. umpquae, and hardhead Mylopharodon conocephalus were examined by using molecular data to investigate monophyly of the genus Ptychocheilus. Phylogenies generated using DNA sequence data from the cytochrome b and 16S ribosomal DNA genes of the mitochondrial genome reveal that Ptychocheilus is a polyphyletic genus and suggest that the taxonomy of the group is in need of further revision. These data yield insights into the evolution of the pikeminnows and help place the significant evolutionary events in context with the geological …

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