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Full-Text Articles in Animal Sciences
Identification Of Parelaphostrongylus Odocoilei (Nematoda: Protostrongylidae) First-Stage Larvae In The Feces Of Gray Wolves (Canis Lupus) By Molecular Methods, Heather M. Bryan, Kathrin A. Sim, Chris T. Darimont, Paul C. Paquet, Brent Wagner, Violeta Muñoz-Fuentes, Judit E. Smits, Nell B. Chilton
Identification Of Parelaphostrongylus Odocoilei (Nematoda: Protostrongylidae) First-Stage Larvae In The Feces Of Gray Wolves (Canis Lupus) By Molecular Methods, Heather M. Bryan, Kathrin A. Sim, Chris T. Darimont, Paul C. Paquet, Brent Wagner, Violeta Muñoz-Fuentes, Judit E. Smits, Nell B. Chilton
Parasitology Collection
First-stage nematode larvae with a dorsal-spine (DSL) were detected in five of 1,565 fecal samples from gray wolves (Canis lupus) collected in British Columbia, Canada, between 2005 and 2008. Molecular techniques were used to identify the DSL because it was not possible to determine their species identity using morphologic characters. The DSL were identified as Parelaphostrongylus odocoilei based on the results of single-strand conformation polymorphism (SSCP) analyses and DNA sequencing of the ribosomal DNA first and second internal transcribed spacers. Finding DSL of P. odocoilei in the feces of gray wolves was unexpected because P. odocoilei adults are parasites of …