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Full-Text Articles in Animal Sciences
Application Of Western Blotting And Enzyme Linked Immunosorbent Assay (Elisa) For The Diagnosis Of Dicrocoelium Dendriticum In Sheep Using Excretory Secretory (E/S) Antigens, Sami̇ Şi̇mşek, Ergün Köroğlu, Armağan Erdem Ütük
Application Of Western Blotting And Enzyme Linked Immunosorbent Assay (Elisa) For The Diagnosis Of Dicrocoelium Dendriticum In Sheep Using Excretory Secretory (E/S) Antigens, Sami̇ Şi̇mşek, Ergün Köroğlu, Armağan Erdem Ütük
Turkish Journal of Veterinary & Animal Sciences
Protein bands of excretory/secretory (E/S) antigens of Dicrocoelium dendriticum were determined using SDS-PAGE and Western blotting. The E/S antigens were prepared from live adult D. dendriticum, which were obtained from slaughtered sheep at a local abattoir. In the SDS-PAGE analysis of D. dendriticum E/S antigen, 9 protein bands with molecular weights varying between 6 and 66 kDa with Coomassie staining and 14 bands between 6 and 205 kDa with silver staining were detected. Blood samples were obtained from sheep at the local abattoir. Following the examination of the organs for D. dendriticum and other helminths, serum samples were divided into …
Changes In Faecal Egg Counts At Different Hours Of The Day And Relationship Between Faecal Egg Count And Parasite Burden In Sheep Naturally Infected With Dicrocoelium Dendriticum, Bayram Şenli̇k, Veli̇ Yilgör Çirak, Mustafa Muz, Recep Tinar
Changes In Faecal Egg Counts At Different Hours Of The Day And Relationship Between Faecal Egg Count And Parasite Burden In Sheep Naturally Infected With Dicrocoelium Dendriticum, Bayram Şenli̇k, Veli̇ Yilgör Çirak, Mustafa Muz, Recep Tinar
Turkish Journal of Veterinary & Animal Sciences
The present study was carried out to determine the variations in the number of eggs excreted in the faeces at different hours of the day and the correlation between faecal egg counts and fluke burden in sheep naturally infected with Dicrocoelium dendriticum. For this purpose faecal samples were taken from 14 sheep at 1-h intervals from 07^{00} to 19^{00}. Faecal samples were examined by modified Benedek sedimentation method and mean egg counts per gram of faeces (EPG) for each hour (average of group) were calculated. In general, egg counts were found higher in faecal samples taken in the afternoon than …