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Full-Text Articles in Animal Sciences
Purification And Characterization Of Glutathione Reductase From Turkey Liver, Pinar Taşer, Mehmet Çi̇ftci̇
Purification And Characterization Of Glutathione Reductase From Turkey Liver, Pinar Taşer, Mehmet Çi̇ftci̇
Turkish Journal of Veterinary & Animal Sciences
This study aimed to purify glutathione reductase (GR) from turkey liver and investigate some of its characteristic features. The purification procedure comprised 2 steps: homogenate preparation and 2',5'-ADP Sepharose 4B affinity gel chromatography. Thanks to the 2 consecutive procedures, the enzyme, having the specific activity of 606.67 EU mg protein^{-1}, was purified with a yield of 10.75% and 2476-fold, and, in order to control enzyme purity, SDS-PAGE was done. Optimal pH, stable pH, optimal temperature, optimal ionic strength, molecular weight, turnover number of enzyme, and k_{cat}/K_M and K_M and V_{max} values for NADPH and glutathione disulfide substrates were also determined …
Histopathological Effects Of Montivipera Xanthina Venom On Rats, Hüseyi̇n Topyildiz, Si̇bel Hayretdağ
Histopathological Effects Of Montivipera Xanthina Venom On Rats, Hüseyi̇n Topyildiz, Si̇bel Hayretdağ
Turkish Journal of Zoology
In this study, the histopathological changes caused by Montivipera xanthina venom to rat skin, skeletal muscle, and liver tissue were analysed by light microscope. The venom (approximately 2.85 mg/kg) was injected intramuscularly into the gastrocnemius muscle of the rats. The animals were dissected 1, 3, and 6 h after the injection time, and tissue samples were taken. Sections 5 µm thick were taken from paraffin blocks of tissue samples, and the sections were stained with haematoxylin and eosin in order to facilitate examination by light microscope. Examinations showed no damage to the epidermis layer of skin. However, damage to the …
Effect Of Season On The Fatty Acid Composition Of The Liver And Muscle Of Alburnus Chalcoides (Güldenstädt, 1772) From Tödürge Lake (Sivas, Turkey), Sali̇h Görgün, Mehmet Ali̇ Akpinar
Effect Of Season On The Fatty Acid Composition Of The Liver And Muscle Of Alburnus Chalcoides (Güldenstädt, 1772) From Tödürge Lake (Sivas, Turkey), Sali̇h Görgün, Mehmet Ali̇ Akpinar
Turkish Journal of Zoology
Seasonal variations in the liver and muscle fatty acid composition of Alburnus chalcoides (Güldenstadt, 1772) from Tödürge Lake (Sivas, Turkey) were determined. The most abundant fatty acids in the investigated seasons and tissues were C16:0, C18:0, C18:1 n-9, C18:1 n-7, C20:4 n-6, C20:5 n-3, and C22:6 n-3. The total saturated fatty acids (SFAs) of the liver and muscle were at the highest in the spring (38.59%) and summer (41.65%), respectively. The total monounsaturated fatty acids (MUFAs) were at the highest in the autumn (51.79%) for liver and in the winter (40.04%) for muscle. The total polyunsaturated fatty acids (PUFAs) were …