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Full-Text Articles in Animal Sciences
A Reference Genome Assembly Of American Bison, Bison Bison Bison, Jonas Oppenheimer, Benjamin D. Rosen, Michael P. Heaton, Brian L. Vander Ley, Wade R. Shafer, Fred T. Schuetze, Brad Stroud, Larry A. Kuehn, Jennifer C. Mcclure, Jennifer P. Barfield, Harvey D. Blackburn, Theodore S. Kalbfleisch, Derek M. Bickhart, Kimberly M. Davenport, Kristen L. Kuhn, Richard E. Green, Beth Shapiro, Timothy P.L. Smith
A Reference Genome Assembly Of American Bison, Bison Bison Bison, Jonas Oppenheimer, Benjamin D. Rosen, Michael P. Heaton, Brian L. Vander Ley, Wade R. Shafer, Fred T. Schuetze, Brad Stroud, Larry A. Kuehn, Jennifer C. Mcclure, Jennifer P. Barfield, Harvey D. Blackburn, Theodore S. Kalbfleisch, Derek M. Bickhart, Kimberly M. Davenport, Kristen L. Kuhn, Richard E. Green, Beth Shapiro, Timothy P.L. Smith
Roman L. Hruska U.S. Meat Animal Research Center: Reports
Bison are an icon of the American West and an ecologically, commercially, and culturally important species. Despite numbering in the hundreds of thousands today, conservation concerns remain for the species, including the impact on genetic diversity of a severe bottleneck around the turn of the 20th century and genetic introgression from domestic cattle. Genetic diversity and admixture are best evaluated at genome-wide scale, for which a high-quality reference is necessary. Here, we use trio binning of long reads from a bison-Simmental cattle (Bos taurus taurus) male F1 hybrid to sequence and assemble the genome of the American plains bison (Bison …
Evaluating Accuracy Of Dna Pool Construction Based On White Blood Cell Counts, Amy N. Abrams, T. G. Mcdaneld, John W. Keele, Carol G. Chitko-Mckown, Larry A. Kuehn, Michael G. Gonda
Evaluating Accuracy Of Dna Pool Construction Based On White Blood Cell Counts, Amy N. Abrams, T. G. Mcdaneld, John W. Keele, Carol G. Chitko-Mckown, Larry A. Kuehn, Michael G. Gonda
Roman L. Hruska U.S. Meat Animal Research Center: Reports
Pooling individual samples prior to DNA extraction can mitigate the cost of DNA extraction and genotyping; however, these methods need to accurately generate equal representation of individuals within pools. The objective of this study was to determine accuracy of pool construction of blood samples based on white blood cell counts compared to two common DNA quantification methods. Fifty individual bovine blood samples were collected, and then pooled with all individuals represented in each pool. Pools were constructed with the target of equal representation of each individual animal based on number of white blood cells, spectrophotometric readings, spectrofluorometric readings, and whole …