Animal Sciences Commons^™

Genetic And Genomic Analysis Of Hyperlipidemia, Obesity And Diabetes Using (C57bl/6j × Tallyho/Jngj) F2 Mice, Taryn P. Stewart, Hyoung Y. Kim, Arnold M. Saxton, Jung H. Kim

Nutrition Publications and Other Works

Background

Type 2 diabetes (T2D) is the most common form of diabetes in humans and is closely associated with dyslipidemia and obesity that magnifies the mortality and morbidity related to T2D. The genetic contribution to human T2D and related metabolic disorders is evident, and mostly follows polygenic inheritance. The TALLYHO/JngJ (TH) mice are a polygenic model for T2D characterized by obesity, hyperinsulinemia, impaired glucose uptake and tolerance, hyperlipidemia, and hyperglycemia.

Results

In order to determine the genetic factors that contribute to these T2D related characteristics in TH mice, we interbred TH mice with C57BL/6J (B6) mice. The parental, F1, and …

Discovery Fall 2010, Misty Bailey

Discovery Research Newsletter

Discovery is a quarterly research newsletter published by the College of Veterinary Medicine Office of Research and Graduate Studies. It is intended to highlight research and sponsored programs in the college and provide information about research opportunities.

Selected Hormonal And Neurotransmitter Mechanisms Regulating, James L. Sartin, Joseph A. Daniel, Brian K. Whitlock, Robyn R. Wilborn

Faculty Publications and Other Works -- Large Animal Clinical Sciences

Appetite control is a major issue in normal growth and in suboptimal growth performance settings. A number of hormones, in particular leptin, activate or inhibit orexigenic or anorexigenic neurotransmitters within the arcuate nucleus of the hypothalamus, where feed intake regulation is integrated. Examples of appetite regulatory neurotransmitters are the stimulatory neurotransmitters neuropeptide Y (NPY), agouti-related protein (AgRP), orexin and melanin-concentrating hormone and the inhibitory neurotransmitter, melanocyte-stimulating hormone (MSH). Examination of messenger RNA (using in situ hybridization and realtime PCR) and proteins (using immunohistochemistry) for these neurotransmitters in ruminants has indicated that physiological regulation occurs in response to fasting for several …

Changes In Plasma Concentrations Of Leptin In Ewes During Pregnancy, Joseph A. Daniel, A B. Milam, M E. Gafnea, Brian K. Whitlock, D H. Keisler

Animal Science Publications and Other Works

Previous research has demonstrated circulating concentrations of leptin increase in ewes during mid pregnancy then decline in late pregnancy and early lactation. The objective of this study was to more narrowly define the timing of changes in circulating concentrations of leptin with pregnancy in ewes. Katahdin ewes (n= 19) located at latitude 34.275 and longitude -85.183 (Mount Berry, GA) were utilized. Blood samples were collected weekly via jugular veinpuncture beginning immediately before ram exposure on September 23 and continuing until 4 weeks post-lambing. Ewes were exposed to a ram fitted with a marking harness for a 63 day breeding season. …

Effect Of Leukemia Inhibitory Factor On Feed Intake And Body Temperature In Sheep, James L. Sartin, D L. Marks, Brian K. Whitlock, Joseph A. Daniel, Barbara P. Steele

Animal Science Publications and Other Works

Leukemia inhibitory factor (LIF) has been suggested to function as a potent inhibitor of feed intake in rodents. These studies were designed to determine whether LIF was found in the ovine hypothalamus and whether LIF inhibited feed intake in sheep. Sheep hypothalami were used to clone LIF to indicate presence of the gene in the hypothalamus. The sequence was similar to published data. Another group of sheep were provided intraventricular (ICV) cannulas and injected with doses of LIF at 250, 500, 1000 and 2500 ng per sheep, ICV. Feed intake was inhibited by the 1000 and 2500 ng dose (trt, …

Changes In Plasma Concentrations Of Growth Hormone And Luteinizing Hormone In Ewes Following Central And Peripheral Treatment With Kisspeptin, Brian K. Whitlock, Joseph A. Daniel, Barbara P. Steele, James L. Sartin

Animal Science Publications and Other Works

Kisspeptin (KP), a neuroendocrine regulator of gonadotropin releasing hormone, has been hypothesized as an integrator of nutrition and hormones critical to metabolism and regulation of reproduction. Recent evidence suggests growth hormone (GH) secretion may be influenced by KP. The objective of this study was to determine if the GH stimulatory effect of KP is due to actions on the hypothalamus or anterior pituitary gland in ewes. Adult ovariectomized ewes (n=8) were fitted with intracerebroventricular (ICV) cannula to facilitate central administration of experimental treatments. Ewes received one of eight treatments [four intravenously (IV) and four ICV]. Peripheral treatments [0 (Veh), 100, …

F758-Forage And Grain Testing Information Sheet, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

No abstract provided.

W156-Clinical Mastitis Evaluation, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

No abstract provided.

W155-Clinical Mastitis And Treatment Record By Time Period, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

No abstract provided.

W154-Clinical Mastitis And Treatment Record By Cow, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

No abstract provided.

W153-Forms Of Mastitis, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

No abstract provided.

W152-Estimating Losses Using Somatic Cell Counts, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

No abstract provided.

W151-Bulk Tank Milk Quality Parameters, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

Bacteria in milk, whether originating from the cow or from the environment, significantly impact the quality of dairy products and therefore consumer acceptance. Bacteria produce heatstable enzymes that can damage milk fat and milk protein. These enzymes are not affected by pasteurization and continue to cause damage in the final dairy product. High bacteria levels in raw milk result in off-flavors, bitter flavors, rancidity and reduced shelf life. The Pasteurized Milk Ordinance specifies safety standards of Grade A milk to protect public health, not to maximize product quality and shelf life. The PMO only sets limits on somatic cell count …

W150-Using Milk Somatic Cell Count Information, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

The level of mastitis infection in a dairy herd can have a significant impact on herd profitability. Losses due to mastitis include decreased milk production, increased treatment costs, discarded milk, premature culling, death, decreased genetic potential, decreased reproductive performance, load rejection due to violation of somatic cell counts (SCC) or antibiotic residues and loss of milk quality premiums (3, 9, 12). Most producers easily relate clinical mastitis with these losses. However, subclinical (or hidden) mastitis may actually cause more losses in a herd than clinical mastitis. Since the animal does not exhibit typical symptoms of the disease, the problem is …

W149-Bulk Tank Milk Quality, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

Who is responsible for the safety and quality of dairy products? Dairy producers? Processors? Retailers? Consumers? In fact, all of these parties – from farm to fork – share in this responsibility. What starts as a high-quality product on the farm can be ruined somewhere along this chain. However, a poor-quality, inferior raw product leaving the farm cannot be transformed into a safe and high-quality product for the consumer. Despite technical advances in milk processing, the safety and quality of dairy products is still determined on the farm. Pasteurization does provide a certain level of safety, but is not the …

W148-Milk Quality And The Tennessee Quality Milk Initiative, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

According to records, in 1995, there were more than 1300 dairy farms in Tennessee. In 2007, the number declined to fewer than 600 dairies (9). There are several reasons for this mass exodus from the dairy industry in Tennessee: low milk prices, shortages of qualified labor, high feed costs, high fertilizer costs, high fuel costs, etc. In addition, many dairy producers in Tennessee and in the Southern Region are at risk of losing their milk market due to below average milk quality. Milk quality continues to be a topic of intense debate in the dairy industry. Dairy producers in the …

W103- Closure & Rehabilitation Of Waste Storage Facilities, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

No abstract provided.

W098-Annual Report Template, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

No abstract provided.

W097-Permit Termination Template, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

No abstract provided.

W096-Name Of Persons And Or Firms Removing Litter, Manure And/Or Process Wastewater From Cafos, The University Of Tennessee Agricultural Extension

Animals/Livestock

No abstract provided.

W095-Agreement For The Removal Of Litter, Manure And/Or Process Wastewater From A Cafo, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

No abstract provided.

W094-Tennessee Phosphorus Risk Index, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

In a waterbody, P is required for algae growth. In most waterbodies, the limited supply of P limits algae growth. Large amounts of P promote rapid growth of large amounts of algae or algae "blooms." This excess algae damages aquatic habitats by reducing sunlight penetration, reducing dissolved oxygen levels and promoting the growth of a toxic form of microscopic marine life. In short, it can result in fish kills, reduce aesthetic value and could make the water less desirable for livestock watering. An example of actions taken to prevent P enrichment of surface water is the lowering of phosphate levels …

W093-Procedures For Manure And Litter Sampling, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

Nutrient composition of manure varies with a number of factors, including animal type, bedding, ration, storage and handling, environmental conditions, field application method, age of manure, timing of sampling and sampling technique. This variability makes book values (or averages) an unreliable source for determining application rates of nitrogen, phosphorus and potassium. Each livestock production operation and manure management system is unique, and an individual farm’s manure analysis can vary from average values by 50 percent or more. Testing manure may better indicate how animal management and other factors actually affect nutrient contents and will allow for more accurate calculation of …

W092-Terminating A Permit, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

A CAFO covered under a general permit must notify the TDEC when the CAFO is no longer in operation. The TDEC retains the right to deny termination of coverage until receipt of the necessary notice and information from the permittee.

CAFO operators should remember that a closure/rehabilitation plan for the waste system was submitted as part of the original permitting process. That closure plan should have met NRCS standards and addressed the maintenance of the facility until proper closure is completed within 360 days. The TDEC may not consider the CAFO closed or terminate the permit until the closure plan …

W091-Annual Report, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

All CAFOs must submit an annual report to TDEC and TDA between January 1 and February 15.

W090-Recordkeeping, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

Recordkeeping is an essential component of a CAFO permit. A producer must keep records current to satisfy the requirements of the regulations. Additionally, without current records, inspectors cannot determine and producers cannot prove the nutrient Management plan (NMP) has been implemented as approved. All records required by the regulation must be maintained for five years, and must be made available to TDEC or EPA upon request.

W089-Discharge Events, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

All wastewater discharges from a CAFO to waters of the state of Tennessee are prohibited. For existing CAFOs, new dairy CAFOs and new cattle CAFOs, an exception will be made when either chronic or catastrophic rainfall events cause an overflow from a facility properly designed, constructed, maintained and operated to contain all process wastewater from the operation (such as wash water, parlor water, watering system overflow, etc.), plus all runoff from a 25-year, 24-hour rainfall event. For new swine and poultry CAFOs, the exception will be made if the facility was properly designed, constructed, maintained and operated to contain all …

W088-Cafo Inspections, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

As a condition of the permit, the producer must allow personnel from the TDEC or the U.S. EPA to inspect the regulated CAFO. They are required to present their credentials and will only inspect a facility “at reasonable times.” This does not mean that they may only inspect the facility at the owner’s convenience. It simply means that most inspections will be conducted during normal business hours. Large CAFOs are required to undergo a yearly inspection at a minimum. Medium CAFOs may be inspected less often.

W087-New Construction On Cafos And Construction Of New Cafos, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

Any liquid waste management system that is constructed, modified, repaired or placed into operation after April 13, 2006 must be designed, constructed, operated and maintained in accordance with final design plans and specifications that meet or exceed standards in the NRCS Field Office Technical Guide and other guidelines as accepted by the Departments of Environment and Conservation, or Agriculture.

W086-Comprehensive Nutrient Management Plans, The University Of Tennessee Agricultural Extension Service

Animals/Livestock

A Comprehensive Nutrient Management Plan (CNMP) is a group of conservation practices and management activities that will ensure both production and natural resource protection goals are achieved. A CNMP will contain actions that address water quality criteria for the feedlot, production area and land on which the manure will be applied. It addresses natural resource concerns dealing with soil erosion, manure and organic by-products, and their potential impacts on water quality.