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Articles 1 - 10 of 10
Full-Text Articles in Animal Sciences
Duplicate Gene Expression In Allopolyploid Gossypium Reveals Two Temporally Distinct Phases Of Expression Evolution, Joshua Udall, Lex E. Flagel, Dan Nettleton, Jonathan F. Wendel
Duplicate Gene Expression In Allopolyploid Gossypium Reveals Two Temporally Distinct Phases Of Expression Evolution, Joshua Udall, Lex E. Flagel, Dan Nettleton, Jonathan F. Wendel
Faculty Publications
Polyploidy has played a prominent role in shaping the genomic architecture of the angiosperms. Through allopolyploidization, several modern Gossypium (cotton) species contain two divergent, although largely redundant genomes. Owing to this redundancy, these genomes can play host to an array of evolutionary processes that act on duplicate genes. Results- We compared homoeolog (genes duplicated by polyploidy) contributions to the transcriptome of a natural allopolyploid and a synthetic interspecific F1 hybrid, both derived from a merger between diploid species from the Gossypium A-genome and D-genome groups. Relative levels of A- and D-genome contributions to the petal transcriptome were determined for 1,383 …
Expression And Evolutionary Relationships Of The Chenopodium Quinoa 11s Seed Storage Protein Gene, Mikel Stevens, Peter J. Maughan, Daniel J. Fairbanks, Marie R. B. Balzotti, Jennifer N. Thornton, David A. Mcclellan, Eric N. Jellen, Craig E. Coleman
Expression And Evolutionary Relationships Of The Chenopodium Quinoa 11s Seed Storage Protein Gene, Mikel Stevens, Peter J. Maughan, Daniel J. Fairbanks, Marie R. B. Balzotti, Jennifer N. Thornton, David A. Mcclellan, Eric N. Jellen, Craig E. Coleman
Faculty Publications
Quinoa (Chenopodium quinoa Willd.) is a food crop cultivated by subsistence farmers and commercial growers on the high Andean plateau, primarily in Bolivia, Peru, and Chile. Present interest in quinoa is due to its tolerance of harsh environments and its nutritional value. It is thought that the seed storage proteins of quinoa, particularly the 11S globulins and 2S albumins, are responsible for the relatively high protein content and ideal amino acid balance of the quinoa seed. Here we report the genomic and cDNA sequences for two 11S genes representing two orthologous loci from the quinoa genome. Important features of the …
Assessment Of Genetic Diversity In The Usda And Cip-Fao International Nursery Collections Of Quinoa (Chenopodium Quinoa Willd.) Using Microsatellite Markers, S. A. Christensen, D. B. Pratt, C. Pratt, P. T. Nelson, M. R. Stevens, Eric N. Jellen, Craig E. Coleman, Daniel J. Fairbanks, A. Bonifacio, Michael J. Maughan
Assessment Of Genetic Diversity In The Usda And Cip-Fao International Nursery Collections Of Quinoa (Chenopodium Quinoa Willd.) Using Microsatellite Markers, S. A. Christensen, D. B. Pratt, C. Pratt, P. T. Nelson, M. R. Stevens, Eric N. Jellen, Craig E. Coleman, Daniel J. Fairbanks, A. Bonifacio, Michael J. Maughan
Faculty Publications
Quinoa (Chenopodium quinoa Willd.) is a staple food crop for millions of impoverished rural inhabitants of Andean South America where it has been cultivated for millennia. Interest in quinoa, due largely to its superior nutritional characteristics, is fuelling a growing export market and has led to an increased focus on genetic research and the development of quinoa breeding programmes throughout South America. The success of these breeding programmes will rely heavily on the development of core germplasm collections and germplasm conservation. We report the development of a set of fluorescence-tagged microsatellite molecular markers that can be used to characterize genetic …
Spotted Cotton Oligonucleotide Microarrays For Gene Expression Analysis, Joshua Udall, Lex E. Flagel, Foo Chung, Andrew W. Woodward, Ran Hovav, Ryan A. Rapp, Jordan M. Swanson, Jinsuk J. Lee, Alan R. Gingle, Dan Nettleton, Christopher D. Town, Z. Jeffrey Chen, Jonathan F. Wendel
Spotted Cotton Oligonucleotide Microarrays For Gene Expression Analysis, Joshua Udall, Lex E. Flagel, Foo Chung, Andrew W. Woodward, Ran Hovav, Ryan A. Rapp, Jordan M. Swanson, Jinsuk J. Lee, Alan R. Gingle, Dan Nettleton, Christopher D. Town, Z. Jeffrey Chen, Jonathan F. Wendel
Faculty Publications
Microarrays offer a powerful tool for diverse applications plant biology and crop improvement. Recently, two comprehensive assemblies of cotton ESTs were constructed based on three Gossypium species. Using these assemblies as templates, we describe the design and creation and of a publicly available oligonucleotide array for cotton, useful for all four of the cultivated species. Results: Synthetic oligonucleotide probes were generated from exemplar sequences of a global assembly of 211,397 cotton ESTs derived from <50 different cDNA libraries representing many different tissue types and tissue treatments. A total of 22,787 oligonucleotide probes are included on the arrays, optimized to target the diversity of the transcriptome and previously studied cotton genes, transcription factors, and genes with homology to Arabidopsis. A small portion of the oligonucleotides target unidentified protein coding sequences, thereby providing an element of gene discovery. Because many oligonucleotides were based on ESTs from fiber-specific cDNA libraries, the microarray has direct application for analysis of the fiber transcriptome. To illustrate the utility of the microarray, we hybridized labeled bud and leaf cDNAs from G. hirsutum and demonstrate technical consistency of results. Conclusion: The cotton oligonucleotide microarray provides a reproducible platform for transcription profiling in cotton, and is made publicly available through http://cottonevolution.info.
Genetic Diversity And Low Reproductive Success In Isolated Populations Of Utah Juniper (Juniperus Osteosperma, Cupressaceae), Loreen Allphin, Val J. Anderson, Amy F. Hunt
Genetic Diversity And Low Reproductive Success In Isolated Populations Of Utah Juniper (Juniperus Osteosperma, Cupressaceae), Loreen Allphin, Val J. Anderson, Amy F. Hunt
Faculty Publications
Utah juniper (Juniperus osteosperma) has greatly expanded its historical range in the western United States. Management plans for the species have focused on curtailing its encroachment into sagebrush and grassland communities. These plans often include burning or other methods of elimination. These methods may result in subdivision or fragmentation of existing juniper stands. We initiated a study at Dugway Proving Ground, a U.S. Army facility, to examine the effects of fragmentation on the reproductive success of Utah juniper in isolated populations. We used enzyme electrophoresis to quantify genetic variability in isolated populations. We also determined population reproductive success by examining …
Evolution Of The Nanog Pseudogene Family In The Human And Chimpanzee Genomes, Daniel J. Fairbanks, Peter J. Maughan
Evolution Of The Nanog Pseudogene Family In The Human And Chimpanzee Genomes, Daniel J. Fairbanks, Peter J. Maughan
Faculty Publications
The NANOG gene is expressed in mammalian embryonic stem cells where it maintains cellular pluripotency. An unusually large family of pseudogenes arose from it with one unprocessed and ten processed pseudogenes in the human genome. This article compares the NANOG gene and its pseudogenes in the human and chimpanzee genomes and derives an evolutionary history of this pseudogene family. Results: The NANOG gene and all pseudogenes except NANOGP8 are present at their expected orthologous chromosomal positions in the chimpanzee genome when compared to the human genome, indicating that their origins predate the human-chimpanzee divergence. Analysis of flanking DNA sequences demonstrates …
Genetic Variation In Ustilago Bullata: Molecular Genetic Markers And Virulence On Bromus Tectorum Host Lines, Mikel Stevens, Daniel J. Fairbanks, Susan E. Meyer, David L. Nelson, Suzette Clement, Jennifer Waters
Genetic Variation In Ustilago Bullata: Molecular Genetic Markers And Virulence On Bromus Tectorum Host Lines, Mikel Stevens, Daniel J. Fairbanks, Susan E. Meyer, David L. Nelson, Suzette Clement, Jennifer Waters
Faculty Publications
The pathogen Ustilago bullata often causes epidemic levels of head smut disease in Intermountain populations of the inbreeding annual grass Bromus tectorum. We examined patterns of genetic variation for virulence and for amplified fragment length polymorphism (AFLP) markers in four U. bullata populations on B. tectorum in northern Utah and southern Nevada. Patterns of disease incidence generally supported a genefor-gene model for virulence and resistance in this pathosystem. Most host lines were susceptible to most or all paired isolates included in cross-inoculation tests, but we found evidence for the existence of four avirulence genes. Host lines with alleles conferring resistance …
Seasonal Acceptance Of Fourwing Saltbush By Sheep When Crested Wheatgrass Is The Alternative, Val J. Anderson, R. D. Horrocks, Christine W. Royer, Stephen B. Monsen
Seasonal Acceptance Of Fourwing Saltbush By Sheep When Crested Wheatgrass Is The Alternative, Val J. Anderson, R. D. Horrocks, Christine W. Royer, Stephen B. Monsen
Faculty Publications
Many sagebrush-grass ranges have been seeded to crested wheatgrass [Agropyron desertorum (Fischer ex Link) Shultes]. These ranges are generally nutritionally inadequate for sheep (Ovis aries L.), except for short grazing periods in the spring and fall. To increase production and diversity, particularly crude protein for late-season grazing, fourwing saltbush [Atriplex canescens (Pursch.) Nutt.] was planted in an existing stand of crested wheatgrass. Quantification of sheep forage preferences on these improved ranges aids in determining the length of the grazing season and the extent to which shrubs provide the supplemental nutrition required. This seasonal grazing study was conducted on a characteristic …
Using Hydrothermal Time Concepts To Model Seed Germination Response To Temperature Dormancy Loss And Priming Effects In Elymus Elymoides, Phil S. Allen, Susan E. Meyer, Susan B. Debaene-Gill
Using Hydrothermal Time Concepts To Model Seed Germination Response To Temperature Dormancy Loss And Priming Effects In Elymus Elymoides, Phil S. Allen, Susan E. Meyer, Susan B. Debaene-Gill
Faculty Publications
Hydrothermal time (HTT) describes progress toward seed germination under various combinations of incubation water potential (Ψ) and temperature (T). To examine changes in HTT parameters during dormancy loss, seeds from two populations of the bunchgrass Elymus elymoides were incubated under seven temperature regimes following dry storage at 10, 20 and 30 °C for intervals from 0 to 16 weeks. Fully after-ripened seeds were primed for 1 week at a range of s. Data on germination rate during priming were used to obtain a HTT equation for each seed population, while data obtained following transfer to water were used to calculate …
Stomatal Characterization Of Grass Leaves By Four Preparation Techniques, Val J. Anderson, John S. Gardner, Joyce Phillips Hardy
Stomatal Characterization Of Grass Leaves By Four Preparation Techniques, Val J. Anderson, John S. Gardner, Joyce Phillips Hardy
Faculty Publications
Four leaf preparation techniques (air drying, tetramethylsilane air drying, critical point drying, and freeze substitution) used in scanning electron microscopy (SEM) were evaluated with respect to the degree of cellular distortion they produce in stomatal guard cells of leaves of Dactylis glomerata and Elymus canadensis. Surface morphological distortion and cuticle disruption in the air-dried and tetramethylsilane air-dried leaves, and cuticle disruption within the critical point-dried tissue made it difficult to obtain measurements.The freeze-substituted tissue experienced little cuticle disturbance, and the cellular morphology appeared normal. The length of the guard cells did not significantly differ between the air-dried, tetramethylsilane air-dried, critical …