Animal Sciences Commons^™

Evaluation Of In Vitro Gas Production And Rumen Bacterial Populations Fermenting Corn Milling (Co)Products, W. L. Williams, L. O. Tedeschi, Paul J. Kononoff, T. R. Callaway, S. E. Dowd, K. Karges, M. L. Gibson

Department of Animal Science: Faculty Publications

The objective of this study was to evaluate the fermentation dynamics of 2 commonly fed corn (co)products in their intact and defatted forms, using the in vitro gas production (IVGP) technique, and to investigate the shifts of the predominant rumen bacterial populations using the 16S rDNA bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP) technique. The bTEFAP technique was used to determine the bacterial profile of each fermentation time at 24 and 48 h. Bacterial populations were identified at the species level. Species were grouped by substrate affinities (guilds) for cellulose, hemicellulose, pectin, starch, sugars, protein, lipids, and lactate. The 2 (co)products …