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Studies Of Protein-Bound Sulfhydryl And Disulfide Groups In The Mitotic Apparatus Of The Sea Urchin, Arbacia Punctulata, Naoko Kawamura
Studies Of Protein-Bound Sulfhydryl And Disulfide Groups In The Mitotic Apparatus Of The Sea Urchin, Arbacia Punctulata, Naoko Kawamura
Doctoral Dissertations
Problems in the present study
From the studies of Sakai and Dan (1959), it became apparent that the changes in soluble SH groups during the first division of sea urchin eggs, as reported by Rapkine (1931), represent the changes in protein SH groups dissolved in 25% TCA. It is also certain from the cytochemical study of the mitotic apparatus (Kawamura and Dan, 1958) that there are abundant SH groups in the protein of spindle and astral fibers. However, there is no evidence to show whether the total amount of protein-bound SH groups (present in both the 25% TCA-soluble and insoluble …