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Full-Text Articles in Animal Sciences
Effects Of Centrifugation And Washing Of Freeze-Thawed Blood On Isolated Dna Characteristics, Mevlüt Arslan
Effects Of Centrifugation And Washing Of Freeze-Thawed Blood On Isolated Dna Characteristics, Mevlüt Arslan
Turkish Journal of Veterinary & Animal Sciences
DNA isolations from the whole blood are commonly performed to obtain DNA for molecular research and diagnostics. Generally, blood samples are taken into anticoagulant tubes and stored in deep freezers until DNA isolation. In fresh blood, pretreatments or leukocytes preparations can be performed and suggested for advanced DNA isolation. However, similar applications in freeze-thawed blood (FTB) have not been shown yet. In the study, centrifugation and washing of FTB were applied as pretreatment before DNA isolations, and their effects on isolated DNA characteristics including DNA integrity, quality, quantity, mitochondrial (mt), and nuclear (n) DNA levels were investigated. Microscopic and flow …
A Comparative Study Of Blood And Milk Chemiluminescence In Healthy Cows And Withsubclinical And Clinical Mastitis, Radoslaw Skorzewski, Wieslaw Krumrych, Hanna Markiewicz, Elzbieta Sandurska
A Comparative Study Of Blood And Milk Chemiluminescence In Healthy Cows And Withsubclinical And Clinical Mastitis, Radoslaw Skorzewski, Wieslaw Krumrych, Hanna Markiewicz, Elzbieta Sandurska
Turkish Journal of Veterinary & Animal Sciences
The aim of the study was to evaluate the luminol-dependent spontaneous and stimulated chemiluminescence (CL) of whole blood and milk in cows with subclinical and clinical mastitis. The research was carried out on 108 Holstein-Friesian dairy cows aged 3-5 years. Based on the assessment of somatic cell count (SCC) in quarter milk, the animals were divided into 3 groups: I - clinically healthy cows (n = 35), II - animals with subclinical mastitis (n = 30) and III - cows with clinical mastitis (n = 43). The material for the research was whole blood and quarter milk. The samples were …
Breed, Age, And Sex-Related Variations In Hematological And Some Biochemicalparameters In The Tornjak Dog, Ivona Alilovic, Dunja Rukavina, Atifa Ajanovic, Cazim Crnkic, Husein Ohran, Amir Zahirovic
Breed, Age, And Sex-Related Variations In Hematological And Some Biochemicalparameters In The Tornjak Dog, Ivona Alilovic, Dunja Rukavina, Atifa Ajanovic, Cazim Crnkic, Husein Ohran, Amir Zahirovic
Turkish Journal of Veterinary & Animal Sciences
There is an increasing significance for the breed, age, and sex-related variations in hematological and biochemical parameters in veterinary medicine. The primary objective of this study was to identify possible breed-related differences and to determine the effect of age and sex on hematologic and serum biochemical parameters in the Bosnian and Herzegovinian - Croatian Shepherd dog Tornjak raised in Bosnia and Herzegovina (B&H). Data from 19 hematological and 17 serum biochemical parameters were analyzed in the group of 100 dogs (47 females and 53 males), sexually intact and aged between one month and twelve years. Breed affected TP (total protein) …
Analysis Of Some Heavy Metals (Cd And Pb) In The Şanlıurfa Province Using Feral Pigeonblood Samples, Özlem Ütme, Füsun Temamoğullari
Analysis Of Some Heavy Metals (Cd And Pb) In The Şanlıurfa Province Using Feral Pigeonblood Samples, Özlem Ütme, Füsun Temamoğullari
Turkish Journal of Veterinary & Animal Sciences
Birds serve as good biomonitors in determining environmental pollution. As for indicators of environmental pollution, we detected blood heavy metal levels in 32 female and 32 male local Feral pigeons in the central districts of Şanlıurfa, located in southeastern Turkey, in one month of each season. The samples were analyzed by coupled plasma-mass spectrometry. Lead (Pb) levels were measured as 233.1 ± 25.7 μg/kg for Haliliye, 101.2 ± 29.7 μg/kg for Karaköprü, 81.3 ± 22.7 μg/kg for Eyyübiye, and 48.1 ± 27.1 μg/ kg for Suruç, whereas cadmium (Cd) levels were measured as 30.6 ± 12.6 μg/kg for Haliliye, 35.7 …
Hematological Effects Of Etomidate And Tricaine In Common Carp, Malgorzata Witeska, Beata Teodorczuk, Katarzyna Lugowska
Hematological Effects Of Etomidate And Tricaine In Common Carp, Malgorzata Witeska, Beata Teodorczuk, Katarzyna Lugowska
Turkish Journal of Veterinary & Animal Sciences
Hematological effects of etomidate and tricaine at concentrations causing general anesthesia or sedation of carp juveniles were compared. Recovery of body balance took similar time in the case of both anesthetics, but fish treated with etomidate were depressed for several hours, while those subjected to tricaine immediately regained normal behavior. Immediately after exposure anesthetics caused minor hematological changes; the only statistically significant effects (decrease of hematocrit and plasma glucose) occurred after sedation with etomidate. One week after exposure a significant increase in mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration occurred in all groups of fish compared to the previous …
The Effect Of Live Saccharomyces Cerevisiae Yeast In The Diet Of Rams On The Digestibility Of Nutrients, Nitrogen And Mineral Retention, And Blood Serum Biochemical Parameters, Barbara Kowalik, Jacek Skomial, Renata Miltko, Malgorzata Majewska
The Effect Of Live Saccharomyces Cerevisiae Yeast In The Diet Of Rams On The Digestibility Of Nutrients, Nitrogen And Mineral Retention, And Blood Serum Biochemical Parameters, Barbara Kowalik, Jacek Skomial, Renata Miltko, Malgorzata Majewska
Turkish Journal of Veterinary & Animal Sciences
The effect of live Saccharomyces cerevisiae yeast added to the diet of rams on the digestibility of carbohydrates and protein in the total tract, retention of nitrogen and mineral elements, and concentration of biochemical parameters in blood serum was examined. The experiment was carried out on 12 Corriedale rams allocated to 2 groups of 6 animals each. The control group was fed meadow hay (45.4%), concentrate (53.2%), and a vitamin?mineral mixture (1.4%), while the second group was fed the same diet supplemented with 2 g day-1 live S. cerevisiae. Adaptation to feeding lasted 21 days; the sampling period lasted 7 …
Blood Chemistry, Thyroid Hormones, And Insulin Serum Content In Bulls Fed A Ration Limited In Energy, Adam Oler, Beata Glowinska
Blood Chemistry, Thyroid Hormones, And Insulin Serum Content In Bulls Fed A Ration Limited In Energy, Adam Oler, Beata Glowinska
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine blood biochemical profile and selected hormone concentrations in bulls fed a diet limited in energy content. Bulls in the experimental group (I) over the 63 days prior to slaughter were provided with ration energy limited to 80% of the maintenance requirement. Animals in the control group (II) received a balanced ration. Blood samples from all of the animals were collected 3 times (A, B, C). The decrease in the ration energy level did not have a significant effect on the concentrations of glucose, total protein, total cholesterol, high-density lipoproteins, triglycerides, and the …
Influence Of Blood Storage Time On Viral Rna Extraction For The Detection Of Bovine Viral Diarrhea Virus In Persistently Infected Cattle, Yingxia Liu, Jie Cao, Junjie Zhang, Kai Huang, Changming Qi
Influence Of Blood Storage Time On Viral Rna Extraction For The Detection Of Bovine Viral Diarrhea Virus In Persistently Infected Cattle, Yingxia Liu, Jie Cao, Junjie Zhang, Kai Huang, Changming Qi
Turkish Journal of Veterinary & Animal Sciences
This study aimed to determine the maximum permissible storage times of blood and serum infected with bovine viral diarrhea virus (BVDV). Viral RNA was successfully extracted from blood and serum that were stored at room temperature (RT) for 7 days and was detected by 1-step RT-PCR. The results of this study demonstrate that BVDV-infected blood can be stored at RT for 7 days and that serum can be stored for 10 days without influencing the viral RNA extraction for the detection of BVDV.
The Effects Of Ichthyophthiriasis On Some Haematological Parameters In Common Carp, Malgorzata Witeska, Elzbieta Kondera, Katarzyna Lugowska
The Effects Of Ichthyophthiriasis On Some Haematological Parameters In Common Carp, Malgorzata Witeska, Elzbieta Kondera, Katarzyna Lugowska
Turkish Journal of Veterinary & Animal Sciences
The present study examined the haematological effects of ichthyophthiriasis in common carp. Blood was collected from 4 groups of juvenile carp: 1. healthy; 2. with minor symptoms of ichthyophthiriasis; 3. with severe symptoms of ichthyophthiriasis (moribund); survivors of ichthyophthiriasis after a 3-week recovery period. Blood was subjected to a standard haematological procedure and the following parameters were evaluated: haematocrit (Ht), haemoglobin concentration (Hb), erythrocyte (RBC) and leukocyte (WBC) counts, mean cell volume (MCV), mean cell haemoglobin (MCH), and mean cell haemoglobin concentration (MCHC). The differential leukocyte count and thrombocyte count were calculated using stained blood smears. No change in Ht …
The Effects Of Dietary Inclusion Of Hydrated Aluminosilicate On Performance And Biochemical Parameters Of Broiler Chickens, Dejan Prvulovic, Danijela Kojic, Gordana Grubor-Lajsic, Slavica Kosarcic
The Effects Of Dietary Inclusion Of Hydrated Aluminosilicate On Performance And Biochemical Parameters Of Broiler Chickens, Dejan Prvulovic, Danijela Kojic, Gordana Grubor-Lajsic, Slavica Kosarcic
Turkish Journal of Veterinary & Animal Sciences
This study investigated the effects of dietary supplementation with hydrated aluminosilicate (antitoxic nutrient-ATN), based on zeolitic ore (of > 90% clinoptilolite), bentonite (of > 83% montmorillonite), and small amounts of activated charcoal, on performance, hematological, serum, and liver biochemical parameters, as well as organ weights and meat quality in broiler chickens. The study included 300 1-day-old broilers of both sexes, which were assigned at random to 2 groups based on treatment: no dietary supplementation (control) and supplementation with 5 g/kg of ATN (ATN group). Dietary supplementation with 5 g/kg of ATN in broiler diets significantly increased weight gain only during the 1{st} …
Correlation Between Biochemical Parameters And Production Traits In Merino Crosses Sheep I- Erythrocyte Potassium And Glutathione, Ni̇hat Mert, Handan Gündüz, Vedat Akgündüz, Münevver Akgündüz
Correlation Between Biochemical Parameters And Production Traits In Merino Crosses Sheep I- Erythrocyte Potassium And Glutathione, Ni̇hat Mert, Handan Gündüz, Vedat Akgündüz, Münevver Akgündüz
Turkish Journal of Veterinary & Animal Sciences
To determine the relationship between biochemical parameters and production traits in sheep, 111 sheep raised in Bandırma Sheep Research Institute were used as living material. Blood samples of 31 Hampshire Down x Merino, 33 Dorset Down x Merino, 27 German Black Headed x Merino and 20 Border Leicester x Merino crosses were taken. The levels and types of blood glutathione (GSH) and erythrocyte potassium (K^+) were determined. Correlations between some production traits such as greasy fleece weight and lamb birth weight were estimated. Statistical significance was found in greasy fleece weight and high glutathione (GSH^H) and low potassium (LK) SBA …
Correlation Between Biochemical Parameters And Production Traits In Merino Cross Sheep Ii- Hemoglobin And Transferrin Types, Ni̇hat Mert, Handan Gündüz, Vedat Akgündüz, Münevver Akgündüz
Correlation Between Biochemical Parameters And Production Traits In Merino Cross Sheep Ii- Hemoglobin And Transferrin Types, Ni̇hat Mert, Handan Gündüz, Vedat Akgündüz, Münevver Akgündüz
Turkish Journal of Veterinary & Animal Sciences
To determine the relationship between biochemical parameters and production traits in sheep, 111 of the animals raised in the Bandırma Sheep Research Institute were used as living materials. The blood samples of 31 Hampshire Down x Merino, 33 Dorset Down x Merino, 27 German Black Headed x Merino and 20 Border Leicester x Merino crosses were taken. Transferrin (Tf) and haemoglobin (Hb) types were determined. Some production traits such as greasy fleece weight, staple length, fiber diameter, ondulance and lamb birth weight were estimated. Important correlations between Hb AB and lamb birth weights (P < 0.05), Hb AB and greasy fleece weight in SBA x Merino crosses (P < 0.05), Hb AB and greasy fleece weight in Border Leicester x Merino crosses (P < 0.05), Tf B type and greasy fleece weight in SBA x Merino and Border Leicester x Merino crosses (P < 0.05) and Tf A type and lamb birth weight in SBA x Merino crosses (P < 0.05) were also determined.
Correlation Between Biochemical Parameters And Production Traits In Merino Cross Sheep Iii-Glucose, Alkaline Phosphatase, Ceruloplasmin, Ni̇hat Mert, Handan Gündüz, Vedat Akgündüz, Münevver Akgündüz
Correlation Between Biochemical Parameters And Production Traits In Merino Cross Sheep Iii-Glucose, Alkaline Phosphatase, Ceruloplasmin, Ni̇hat Mert, Handan Gündüz, Vedat Akgündüz, Münevver Akgündüz
Turkish Journal of Veterinary & Animal Sciences
A group of 111 sheep raised in the Bandırma Sheep Research Institute were used as living materials for this study. Blood samples from 31 Hampshire Down x Merino, 33 Dorset Down x Merino, 27 German Black Headed x Merino and 20 Border Leicester x Merino crosses were taken. Plasma glucose, alkaline phosphatase (ALP) and ceruloplasmin levels were determined. Some production traits such as greasy fleece weight, staple length, fiber diameter, ondulance, lamb birth weight, reproduction traits and the live weight gain of lambs were estimated. There were blood glucose differences between the breeds and an important correlation between greasy fleece …
Effect Of Vitamin E Supplementation On The Level Of Milk And Plasma Vitamin E And Thiobarbituric Acid Levels Of Milk, Ö. Hakan Muğlali, Nurcan Çeti̇nkaya, Ayhan Güler, Uğur Gögüş, Serap Ulutürk
Effect Of Vitamin E Supplementation On The Level Of Milk And Plasma Vitamin E And Thiobarbituric Acid Levels Of Milk, Ö. Hakan Muğlali, Nurcan Çeti̇nkaya, Ayhan Güler, Uğur Gögüş, Serap Ulutürk
Turkish Journal of Veterinary & Animal Sciences
In the study, the effect of various amounts (200 and 400 mg/head) and administration routes (intra-muscular or per-os) of vitamin E on blood and milk vitamin E levels and thiobarbituric acid (TBA) levels of milk was determined. A total of 50 Akkaraman sheep with a control and four treatment groups each containing 10 animal were used in the study. Weekly intra-muscular (IM) injections were administered over two months. Blood samples were collected at 15 day intervals a month after the administration and blood and milk samples were collected weekly for two months after the end of the administrative period. In …
Investigations Of Serratia Liquefaciens Infection In Rainbow Trout (Oncorhynchus Mykiss Walbaum), Seyi̇t Aydin, Zekai̇ Erman, Ömer Cevdet Bi̇lgi̇n
Investigations Of Serratia Liquefaciens Infection In Rainbow Trout (Oncorhynchus Mykiss Walbaum), Seyi̇t Aydin, Zekai̇ Erman, Ömer Cevdet Bi̇lgi̇n
Turkish Journal of Veterinary & Animal Sciences
This study was conducted to investigate Serratia liquefaciens infection in juvenile cultured rainbow trout (Oncorhynchus mykiss Walbaum) during June and July in 1996 about 20 days after flooding in three farms in Erzurum. Bacterial isolates were identified from each farm and tested to determine sensitivity against 18 chemotherapeutants. In addition to minimum bactericidal concentrations (MBC) of chloramine-T and potassium permanganate (KmnO4) were exposures of 17.07-20 mg/l for 1 h and 23.27 mg/l for 10 min. Infections caused fallen scales, bloody and swollen kidney, hyperaemic and pale regions in the liver, haemorrhagic spots in the gills and bloody exudate in the …
The Industrial Fluorosis Caused By A Coal-Burning Power Station And Its Effects On Sheep, Ulvi̇ Reha Fi̇danci, Tevhi̇de Sel
The Industrial Fluorosis Caused By A Coal-Burning Power Station And Its Effects On Sheep, Ulvi̇ Reha Fi̇danci, Tevhi̇de Sel
Turkish Journal of Veterinary & Animal Sciences
The aim of this investigation was to examine the industrial fluorosis caused by the coal burning power station in Muğla-Yatağan. Blood and urine samples of sheep, and water, plant and soil samples from around the power station were collected four times throughout the year to cover all four seasons. Fluoride ion concentrations in the water, urine, plant and soil samples were detected potentiometrically using an ion selective electrode. Serum Alkaline phosphatase (ALP-EC 3.1.3.1), Alanine aminotransferase (ALT-EC 2.6.1.2), Aspartate aminotransferase (AST-EC 2.6.1.1) and Lactate dehydrogenase (LDH-EC 1.1.1.27) enzyme activities were analysed spectrophotometrically using Sigma commercial kits. Significantly higher fluoride ion concentration …
Vitamin A, Vitamin E And Selenium Levels In Blood And Milk Sera Of Cattle With Foot And Mouth Disease, Serdar Kizil, Ari̇f Altintaş
Vitamin A, Vitamin E And Selenium Levels In Blood And Milk Sera Of Cattle With Foot And Mouth Disease, Serdar Kizil, Ari̇f Altintaş
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine \beta-carotene, retinol (Vit A), selenium (Se) and \alpha-tocopherol (Vit E) levels in the sera of blood and milk from cattle with foot and mouth disease (FMD). A total of 120 blood sera and 22 milk sera were used for the analysis. \beta-carotene and Vit A, Se and Vit E levels were determined by spectrophotometer, fluorophotometer and HPLC respectively. Serum samples were obtained from 0-1 and 1-3-year-old cattle bred in public and state farms. Animals were grouped according to their sex, vaccination status and health or infection with FMD. Results of the biochemical …
The Monthly Variations In The Blood Glucose Level Of Capoeta Capoeta Capoeta (G.Ldenstaedt, 1772) Living In Aras River, Seyi̇t Aydin, Ayhan Yildirim, Orhan Erdoğan
The Monthly Variations In The Blood Glucose Level Of Capoeta Capoeta Capoeta (G.Ldenstaedt, 1772) Living In Aras River, Seyi̇t Aydin, Ayhan Yildirim, Orhan Erdoğan
Turkish Journal of Veterinary & Animal Sciences
In this study, the monthly variation in the blood glucose level of Capoeta capoeta capoeta caught from Aras between August 1995 and June 1996 was determined. The average blood glucose level was determined to be 112.04±5.19 mg/dl in males and 106.32±9.68 mg/dl in females and 110.06±4.19 mg/dl in the population. The blood glucose level was highest (141.07±13.67 mg/dl) in April, and lowest (69.14±9.75 mg/dl) in September. It was deter- mined that the level of blood glucose was related to temperature and reproduction.
The Seasonal Variation In Blood Glucose Levels In Barbel, Barbus Plebejus Escherichi(Steindachner, 1897), Living In Çoruh Basin-Oltu Stream, Ayhan Yildirim, Mustafa Türkmen, İrfan Altuntaş
The Seasonal Variation In Blood Glucose Levels In Barbel, Barbus Plebejus Escherichi(Steindachner, 1897), Living In Çoruh Basin-Oltu Stream, Ayhan Yildirim, Mustafa Türkmen, İrfan Altuntaş
Turkish Journal of Veterinary & Animal Sciences
In this study, seasonal variation in blood glucose levels in Barbus plebejus escherichi living in Çoruh Basin-Oltu Stream was determined. The average blood glucose levels were observed to be 185.27±15.79 mg/dl in males, 157.05±10.52 mg/dl in females and 174.74±9.04 mg/dl in the total population.
Blood And Milk Protein Polymorphism And Their Effects On Various Production Traits In Simmental Cows, Özel Şekerden, Faruk Doğrul, Hüseyi̇n Erdem
Blood And Milk Protein Polymorphism And Their Effects On Various Production Traits In Simmental Cows, Özel Şekerden, Faruk Doğrul, Hüseyi̇n Erdem
Turkish Journal of Veterinary & Animal Sciences
This research was carried out to determine the transferrin (Tf) and haemoglobine (Hb) and milk protein (alfa-S_1-casein, beta casein, capa casein, beta lactoglobuline) types, and relationships between Tf and Hb types and milk protein genotypes with various characteristics. The relationship between Tf types and milk yields of control milking and control day were only statistically significant (P0.05). None of the investigated characteristics were effected by milk protein genetic variants at statistically significant level.
The Effect Of Formaldehyde And Blood Treatments Of Sunflower Meal On The Degradationcharacteristics And Effective Degradability In Rumen, Sakine Yalçin, Adnan Sehu, Fatma Karakaş
The Effect Of Formaldehyde And Blood Treatments Of Sunflower Meal On The Degradationcharacteristics And Effective Degradability In Rumen, Sakine Yalçin, Adnan Sehu, Fatma Karakaş
Turkish Journal of Veterinary & Animal Sciences
This study was carried out to evaluate the effects of formaldehyde and blood treatments of sunflower meal on the degra-dation characteristics and effective degradability of dry matter(DM), organic matter and crude protein. Sunflower meal treated with 0.8 g formaldehyde/100 g crude protein, 0.5, 0.75, 1.5 and 2 L whole blood/kg DM. Three adult rumen fistulated merino rams were used in this study. Samples were incubated in the rumen for 2, 4, 8, 16, 24, 48 and 60 h. Formaldehyde treatment of sunflower meal reduced the amount of rapidly soluble protein, degradation rate constant and effective degradability of protein by 75.36, …